Praeruptorin A (PA) is one of the active ingredients found in the dried root of Peucedanum praeruptorum Dunn, has been reported to possess anticancer effects against various types of cancer. However, the effect of PA on human hepatocellular carcinoma (HCC) remains uncleared. In this study, our results indicated that PA did not induce cytotoxicity or alter cell cycle distribution in human HCC cells (Huh-7, SK-Hep-1, and PLC/PRF/5 cells). Instead, PA inhibited the migration and invasion of human HCC cells while downregulating the expression of matrix metalloproteinase-1 (MMP1) and activating the extracellular signal-regulated kinase (ERK) signaling pathways. Furthermore, blocking the ERK signaling pathway through siERK restored the expression of MMP1 and the invasive ability of PA-treated HCC cells. In conclusion, our results demonstrate the antimetastatic activity of PA against human HCC cells, supporting its potential as a therapeutic agent of HCC treatments.

中文翻译：

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Praeruptorin A 通过靶向 ERK/MMP1 信号通路减少人肝癌细胞的转移

Praeruptorin A (PA) 是在 Peucedanum praeruptorum Dunn 干燥根中发现的活性成分之一，据报道对各种类型的癌症具有抗癌作用。然而，PA对人肝细胞癌（HCC）的影响仍不清楚。在本研究中，我们的结果表明 PA 不会在人 HCC 细胞（Huh-7、SK-Hep-1 和 PLC/PRF/5 细胞）中诱导细胞毒性或改变细胞周期分布。相反，PA 抑制人 HCC 细胞的迁移和侵袭，同时下调基质金属蛋白酶-1 (MMP1) 的表达并激活细胞外信号调节激酶 (ERK) 信号通路。此外，通过 siERK 阻断 ERK 信号通路恢复了 MMP1 的表达和 PA 处理的 HCC 细胞的侵袭能力。综上所述，