Sensors and Actuators B: Chemical ( IF 8.0 ) Pub Date : 2020-11-23 , DOI: 10.1016/j.snb.2020.129235 Carolyn M Levinn 1 , Michael D Pluth 1
|
Hydrogen sulfide (H2S) is an important biomolecule and significant efforts have focused on developing chemical tools to aid different biological investigations. Of such tools, there are relatively few chemiluminescent or bioluminescent methods for H2S detection. Here we report two dioxetane-based chemiluminescent probes for H2S detection. With these probes, we directly compare the probe response to H2S-mediated azide reduction and nucleophilic displacement of 2,4-dinitrophenyl motifs and demonstrate that the SNAr cleavage of the DNP group results in a larger response and greater stability in water.
中文翻译：
用于检测水中硫化氢的化学发光探针上的触发基序的直接比较
硫化氢 (H 2 S) 是一种重要的生物分子，人们大力致力于开发化学工具来帮助不同的生物学研究。在这些工具中，用于H 2 S检测的化学发光或生物发光方法相对较少。在这里，我们报告了两种用于 H 2 S 检测的基于二氧杂环丁烷的化学发光探针。使用这些探针，我们直接比较了探针对 H 2 S 介导的叠氮化物还原和 2,4-二硝基苯基基序亲核置换的响应，并证明 DNP 基团的 S N Ar 裂解导致更大的响应和在水中更高的稳定性。
京公网安备 11010802027423号