This study investigated the effects of cofactor metabolism on secondary metabolite production in M. purpureus through the application of different cofactor engineering strategies. Total pigment production dramatically increased by 39.08% and 40.89%, and yellow pigment production increased by 74.62% and 114.06% after the addition of 1.0 mg/L of the exogenous cofactor reagents methyl viologen and rotenone, respectively, in submerged batch-fermentation. The extracellular red pigment tone changed to yellow with the application of electrolytic stimulation at 800 mV/cm², but almost no citrinin production was detected. In addition, the total pigment, yellow pigment and citrinin production increased by 35.46%, 54.89% and 6.27% after disruption of the nuoⅠ gene that encodes NADH-quinone oxidoreductase, respectively. Thus, cofactor metabolic engineering strategies could be extended to the industrial production of Monascus pigment or high yellow pigment with free citrinin production.

本研究通过应用不同的辅助因子工程策略研究了辅助因子代谢对紫癜支原体次级代谢产物产生的影响。在浸没式分批发酵中分别添加1.0 mg / L外源性辅因子试剂甲基紫精和鱼藤酮后，总色素产量急剧增加了39.08％和40.89％，黄色色素产量增加了74.62％和114.06％。通过在800 mV / cm ²的电解刺激下，细胞外红色颜料的色调变为黄色，但几乎未检测到柠檬黄素的产生。此外，总的颜料，黄色颜料和桔霉素生产中断后增加了35.46％，54.89％和6.27％nuoⅠ该基因分别编码NADH-醌氧化还原酶。因此，辅助因子代谢工程策略可以扩展到工业生产红曲色素或高游离色素的高黄色素。