Polyethylene film is one of the most common types of recalcitrant plastic waste materials. Information regarding the fate of plastic films in soil is scarce compared to the fate of plastic films in aquatic environments. The aim of this study was to evaluate the effects of soil fauna and of impregnation of polyethylene films with oil on the colonization of low-density polyethylene films by prokaryotic communities. The field experiment was performed in a monsoon tropical forest (Vietnam). Polyethylene films were incubated in thermally pre-defaunated soil isolated from the surrounding soil by a stainless steel mesh. Three mesh sizes were used, allowing access to different size groups of soil fauna. The diversity, taxonomic structure and co-occurrence patterns in prokaryotic communities were studied using high-throughput sequencing of 16S rRNA gene libraries. The prokaryotic communities that formed on polyethylene films were slightly different from those inhabiting the surrounding soil. Contrary to our expectations, no difference in the diversity of prokaryotes was observed between microcosms with different mesh sizes. Oil impregnation also had only a minor influence on the prokaryotic community structure. Polyethylene films incubated in microcosms with soil appeared to be colonized by various consortia of prokaryotes as a barren and inert surface.

聚乙烯薄膜是最顽固的塑料废料之一。与水生环境中塑料薄膜的命运相比，关于土壤中塑料薄膜的命运的信息很少。这项研究的目的是评估土壤动物和用油浸渍聚乙烯薄膜对原核生物群落对低密度聚乙烯薄膜定殖的影响。田间试验是在季风热带森林（越南）中进行的。聚乙烯薄膜在通过不锈钢网与周围土壤隔离的热预分解土壤中孵育。使用了三种网格大小，可以访问不同大小的土壤动物群。多样性 使用16S rRNA基因文库的高通量测序研究了原核生物群落的分类结构和共现模式。在聚乙烯膜上形成的原核生物群落与居住在周围土壤中的生物略有不同。与我们的预期相反，在具有不同筛目尺寸的微观世界之间未观察到原核生物多样性的差异。油浸对原核生物群落结构的影响也很小。在与土壤的缩影中孵育的聚乙烯薄膜似乎被原核生物的各种聚生体定殖为贫瘠和惰性的表面。在不同网孔尺寸的缩影之间未观察到原核生物多样性的差异。油浸对原核生物群落结构的影响也很小。在与土壤的缩影中孵育的聚乙烯薄膜似乎被原核生物的各种聚生体定殖为贫瘠和惰性的表面。在不同网孔尺寸的缩影之间未观察到原核生物多样性的差异。油浸对原核生物群落结构的影响也很小。在与土壤的缩影中孵育的聚乙烯薄膜似乎被原核生物的各种聚生体定殖为贫瘠和惰性的表面。