In this work, two new hybrid benzothiadiazole-coumarin derivatives have been designed and applied as selective live cell fluorescence imaging probes. The developed dyes had their photoproperties evaluated and proved to be stable emitters. DFT calculations have been used to shed some light on the photophysical properties observed for both fluorophores and explained the obtained data. The lipophilic structures selectively stained the plasma membrane of both live and fixed cells of several tumoral and healthy lineages. Comparison of the novel hybrid fluorogenic dyes with the commercially available CellMask proved the preference for plasma membrane staining of the dyes. Finally, zebrafish embryos have been used as complexes models and these embryos were selectively stained, thus indicating the advantages of the hybrid dyes also for multicellular staining in comparison with the commercial dye.

在这项工作中，已经设计了两种新的杂合苯并噻二唑-香豆素衍生物，并将其用作选择性活细胞荧光成像探针。对开发的染料进行了光性能评估，并证明是稳定的发射体。DFT计算已被用于阐明两种荧光团的光物理性质，并解释了获得的数据。亲脂性结构选择性地染色了几种肿瘤和健康谱系的活细胞和固定细胞的质膜。新型杂化荧光染料与市售CellMask的比较证明了该染料对质膜染色的偏爱。最后，斑马鱼胚胎已被用作复合体模型，并对这些胚胎进行了选择性染色，