Providing robust affinity for HRP to avoid leakage and loss of activity, and improve resistance to external environments is essential to fabricate HRP-functionalized surface applied in various biosensors. Here, a facile universal polydopamine coating is prepared via dopamine self-polymerization process on mesoporous magnetic nanocrystal clusters. It is found that the adsorption of mesoporous nanocomposites for horseradish peroxidase (HRP) is pH dependent, and the high binding capacity toward HRP is mainly based on catecholic anchoring at pH ≤ 8. The interaction is stable even when exposed to a wide range of pH from 4 to 11 with negligible leakage rate below 7%. The immobilized HRP can effectively resist the influence of surrounding environments such as acid, alkali, high salt and high temperature on the catalytic activity. The nanocomposites are further used to measure glucose in human serum with good reliability.

为HRP提供强大的亲和力以避免泄漏和活性损失，并提高对外部环境的抵抗力，对于制造应用于各种生物传感器的HRP功能化表面至关重要。在这里，通过多巴胺自聚合过程在介孔磁性纳米晶体簇上制备了一种方便的通用聚多巴胺涂层。发现介孔纳米复合材料对辣根过氧化物酶（HRP）的吸附取决于pH，对HRP的高结合能力主要是基于在pH≤8时的锚定锚固。即使暴露于广泛的pH范围，相互作用也是稳定的从4到11，泄漏率可忽略不超过7％。固定化的HRP可以有效地抵抗酸，碱，高盐和高温等周围环境对催化活性的影响。