There is growing evidence that microRNA-148b (miR-148b) can inhibit the growth of malignant cells while sirtuin 7 (SIRT7) may perform its carcinogenic effect by deacetylating H3K18. This study investigated the mechanism of miR-148b/SIRT7 on how it affects the malignant biological behavior of melanoma. It was established that the expression of miR-148b was downregulated in melanoma while that of SIRT7 was upregulated but negatively regulated by miR-148b through binding to the 3UTR of SIRT7. Ectopic expression of miR-148b reduced the proliferation, migration, and invasion of melanoma cells, but SIRT7 reversed these functions of miR-148b. Moreover, tumor growth and metastasis experiments showed that miR-148b could significantly suppress proliferation and metastasis of melanoma in vivo. Overall, miR-148b inhibits the malignant biological behavior of melanoma by reducing the expression level of SIRT7. The development of miR-148b as a novel potential therapeutic approach for melanoma may be possible in the future.

中文翻译：

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MicroRNA-148b通过降低Sirtuin 7表达水平抑制黑素瘤的恶性生物学行为

越来越多的证据表明，微小RNA-148b（miR-148b）可以抑制恶性细胞的生长，而瑟土因7（SIRT7）可能通过使H3K18脱乙酰化而发挥其致癌作用。这项研究调查了miR-148b / SIRT7对黑色素瘤的恶性生物学行为的影响机制。已经确定，在黑素瘤中miR-148b的表达下调，而SIRT7的表达上调，但通过与SIRT7的3个UTR结合而被miR-148b负调节。miR-148b的异位表达减少了黑色素瘤细胞的增殖，迁移和侵袭，但SIRT7逆转了miR-148b的这些功能。此外，肿瘤生长和转移实验表明，miR-148b可以在体内显着抑制黑色素瘤的增殖和转移。总体而言，miR-148b通过降低SIRT7的表达水平来抑制黑素瘤的恶性生物学行为。miR-148b作为黑色素瘤的新型潜在治疗方法的开发可能在将来出现。