The natural characteristics of deoxyribonucleic acid (DNA) enable its advanced applications in nanotechnology as a special tool that can be detected by high-resolution imaging with precise localization. Super-resolution (SR) microscopy enables the examination of nanoscale molecules beyond the diffraction limit. With the development of SR microscopy methods, DNA nanostructures can now be optically assessed. Using the specific binding of fluorophores with their target molecules, advanced single-molecule localization microscopy (SMLM) has been expanded into different fields, allowing wide-range detection at the single-molecule level. This review discusses the recent progress in the SR imaging of DNA nano-objects using SMLM techniques, such as direct stochastic optical reconstruction microscopy, binding-activated localization microscopy, and point accumulation for imaging nanoscale topography. Furthermore, we discuss their advantages and limitations, present applications, and future perspectives.

中文翻译：

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随机切换定位显微镜的单DNA分子传感器的向上亚衍射成像

脱氧核糖核酸（DNA）的自然特性使其可以作为特殊工具在纳米技术中得到先进的应用，可以通过高分辨率成像和精确定位来检测脱氧核糖核酸。超分辨率（SR）显微镜可以检查超出衍射极限的纳米级分子。随着SR显微镜方法的发展，现在可以光学评估DNA纳米结构。利用荧光团与目标分子的特异性结合，高级单分子定位显微镜（SMLM）已扩展到不同领域，从而可以在单分子水平进行宽范围检测。这篇综述讨论了使用SMLM技术对DNA纳米物体进行SR成像的最新进展，例如直接随机光学重建显微镜，结合激活的定位显微镜，和点累积以成像纳米级地形。此外，我们讨论了它们的优点和局限性，当前的应用程序以及未来的前景。