The natural characteristics of deoxyribonucleic acid (DNA) enable its advanced applications in nanotechnology as a special tool that can be detected by high-resolution imaging with precise localization. Super-resolution (SR) microscopy enables the examination of nanoscale molecules beyond the diffraction limit. With the development of SR microscopy methods, DNA nanostructures can now be optically assessed. Using the specific binding of fluorophores with their target molecules, advanced single-molecule localization microscopy (SMLM) has been expanded into different fields, allowing wide-range detection at the single-molecule level. This review discusses the recent progress in the SR imaging of DNA nano-objects using SMLM techniques, such as direct stochastic optical reconstruction microscopy, binding-activated localization microscopy, and point accumulation for imaging nanoscale topography. Furthermore, we discuss their advantages and limitations, present applications, and future perspectives.

中文翻译：

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基于随机切换定位显微镜的单 DNA 分子传感器的前向亚衍射成像


脱氧核糖核酸 (DNA) 的天然特性使其能够作为一种特殊工具在纳米技术中得到先进应用，可以通过高分辨率成像和精确定位进行检测。超分辨率 (SR) 显微镜能够检查超出衍射极限的纳米级分子。随着 SR 显微镜方法的发展，现在可以对 DNA 纳米结构进行光学评估。利用荧光团与其目标分子的特异性结合，先进的单分子定位显微镜（SMLM）已扩展到不同的领域，可以在单分子水平上进行广泛的检测。本综述讨论了使用 SMLM 技术对 DNA 纳米物体进行 SR 成像的最新进展，例如直接随机光学重建显微镜、结合激活定位显微镜和用于成像纳米级形貌的点积累。此外，我们还讨论了它们的优点和局限性、当前的应用和未来的前景。