Six commercial biocontrol agents (BCAs: Aureobasidium pullulans, Bacillus amyloliquefaciens, B. amyloliquefaciens plantarum, B. subtilis, Metschnikowia fructicola, or Trichoderma atroviride) were applied to bunch trash that was then incubated at one of five temperatures (T, 15, 20, 25, 30, and 35 °C) and one of five relative humidity levels (RH, 60, 80, 90, 95, and 100%). After 1 to 13 days of incubation (BCA colonization period), the number of colony forming units (CFUs) was assessed. The colonization of bunch trash in response to T/RH conditions and BCA colonization period differed among the BCAs; the coefficients of variation among the BCAs ranged from 104.6 to 397.7%. Equations were developed that accounted for the combined effects of the T, RH, and BCA colonization period on BCA colonization of bunch trash. Assuming that the equations, which had an R² > 0.87, correctly predict BCA growth under field conditions, they would help farmers select the BCA to be used for a specific application based on weather conditions at the time of treatment and in the following days. The equations would also help predict how long an early season BCA application remains effective and thereby help farmers decide whether and when a second BCA application may be needed.

中文翻译：

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温度和湿度影响下微生物对葡萄串垃圾的定殖对灰葡萄孢的生物防治

6名商业生物防治剂（BCAS：出芽短梗霉，解淀粉芽孢杆菌，解淀粉芽孢杆菌乳杆菌，枯草芽孢杆菌，梅奇，或深绿木霉）放在一堆垃圾上，然后在五个温度（T，15、20、25、30和35°C）之一和五个相对湿度水平（RH，60、80、90、95和95°C之一）下孵育100％）。孵育1至13天后（BCA定殖期），评估菌落形成单位（CFU）的数量。BCAs对T / RH条件和BCA定殖期的响应对垃圾的定殖有所不同。BCA之间的变异系数介于104.6％至397.7％之间。开发了方程，该方程解释了T，RH和BCA定殖期对一堆垃圾的BCA定殖的综合影响。假设方程中有一个R ²> 0.87，可以正确预测田间条件下的BCA增长，这将帮助农民根据处理时和随后几天的天气条件选择用于特定应用的BCA。这些方程式还将有助于预测早期BCA施用仍然有效的时间，从而帮助农民决定是否以及何时需要第二次BCA施用。