The experiment evaluated the influence of different light qualities and 6-benzyladenine (BA) concentration in a medium on the leaf response of multiplied Gerbera jamesonii Bolus ex Hook. f.‘Big Apple’ shoots. Three different light-emitting diode (LED) spectra—100% blue (B), 100% red (R) and red and blue mixture (7:3, RB)—were used, and a fluorescent lamp was used as a control (Fl). Concentrations of BA in Murashige and Skoog (MS) medium were 1, 2.5 and 5 µM. Leaves developed under 100% blue light had a lower frequency of stomata and a smaller area as compared with those from plants exposed to light with red in spectrum. Under 100% red light, the leaf area and the frequency of stomata increased along with growing concentration of BA in the medium. The thickest mesophyll was spotted in the cross-section of leaves exposed to the blue LED light. Leaves developed under the 100% red light had the thinnest mesophyll layers. Increasing concentration of BA in the medium resulted in enhanced leaf blade thickness. The cross-section of leaf vascular bundles was only half of that in petioles. The leaves under the LED combinations had larger vascular bundles than those under fluorescent light. The highest level of photosynthetic pigments was noticed in the leaves grown under LED R and RB lights. Our study demonstrated that 2.5 µM BA and a mixture of blue and red light provided by LED improved leaf quality during multiplication of gerbera shoots.

中文翻译：

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非洲菊腋芽微繁期间叶片对不同光谱成分的响应

实验评估了不同光质和培养基中6-苄基腺嘌呤（BA）浓度对多种非洲菊叶响应的影响Bolus ex Hook。f。“大苹果”笋。使用了三种不同的发光二极管（LED）光谱-100％蓝色（B），100％红色（R）和红色和蓝色混合物（7：3，RB）-，并将荧光灯用作对照（ Fl）。Murashige和Skoog（MS）培养基中BA的浓度为1、2.5和5 µM。与暴露于光谱中红色光的植物相比，在100％蓝光下发育的叶片具有较低的气孔频率和较小的面积。在100％红光下，随着培养基中BA浓度的增加，叶片面积和气孔频率增加。在暴露于蓝色LED灯的叶子横断面上发现最厚的叶肉。在100％红光下发育的叶片具有最薄的叶肉层。培养基中BA浓度的增加导致叶片厚度增加。叶片维管束的横截面仅为叶柄的一半。与荧光灯相比，LED组合下的叶片具有更大的维管束。在LED R和RB光下生长的叶片中发现了最高水平的光合色素。我们的研究表明，在非洲菊的繁殖过程中，2.5 µM BA和LED提供的蓝光和红光的混合物可改善叶片质量。