Abstract Context The Traditional Chinese herb medicine Dalbergia odorifera T. Chen (Fabaceae), exerted a protective effect on myocardial ischaemia. Latifolin is a neoflavonoid extracted from Dalbergia odorifera. It has been reported to have the effects of anti-inflammation and cardiomyocyte protection. Objective To investigate whether latifolin can improve myocardial infarction (MI) through attenuating myocardial inflammatory and to explore its possible mechanisms. Materials and methods Left coronary artery was ligated to induce a rat model of MI, and the rats were treated with sodium carboxymethyl cellulose (CMC-Na) or different doses of latifolin (25, 50, 100 mg/kg/d) by oral gavage for 28 days. Serum contents of myocardial enzyme were measured at seven and fourteen days after treatment. Cardiac function, infarct size, histopathological changes and inflammatory cells infiltration was assessed at 28 days after treatment. Western blotting was used to investigate the underlying mechanisms. Results Latifolin treatment markedly decreased the contents of myocardial enzymes, and increased left ventricular ejection fraction (85.27% vs. 59.11%) and left ventricular fractional shortening (62.71% vs. 45.53%). Latifolin was found to significantly reduced infarction size (27.78% vs. 39.07%), myocardial fibrosis and the numbers of macrophage infiltration (436 cells/mm2 vs. 690 cells/mm2). In addition, latifolin down-regulated the expression levels of hypoxia-inducible factor-1α (0.95-fold), phospho-nuclear factor-κB (0.2-fold) and interleukin-6 (1.11-fold). Discussion and conclusions Latifolin can protect against myocardial infarction by improving myocardial inflammation through the HIF-1α/NF-κB/IL-6 signalling pathway. Accordingly, latifolin may be a promising drug for pharmacological treatment of ischaemic cardiovascular disease.

中文翻译：

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Latifolin 通过 HIF-1α/NF-κB/IL-6 通路减轻心肌炎症来预防心肌梗塞

摘要 背景 中药黄檀（豆科）对心肌缺血有保护作用。Latifolin 是一种从 Dalbergia odorifera 中提取的新黄酮类化合物。据报道具有抗炎和保护心肌细胞的作用。目的探讨latifolin是否能通过减轻心肌炎症来改善心肌梗死（MI），并探讨其可能的机制。材料与方法 结扎左冠状动脉诱导大鼠心肌梗死模型，给予大鼠羧甲基纤维素钠（CMC-Na）或不同剂量的latifolin（25、50、100mg/kg/d）灌胃治疗28 天。在治疗后第7天和第14天测量心肌酶的血清含量。心功能、梗塞面积、在治疗后 28 天评估组织病理学变化和炎症细胞浸润。蛋白质印迹被用来研究潜在的机制。结果 Latifolin治疗显着降低心肌酶含量，增加左心室射血分数（85.27% vs. 59.11%）和左心室缩短分数（62.71% vs. 45.53%）。发现 Latifolin 可显着减少梗死面积（27.78% 对 39.07%）、心肌纤维化和巨噬细胞浸润的数量（436 细胞/mm2 对 690 细胞/mm2）。此外，latifolin 下调缺氧诱导因子-1α（0.95 倍）、磷酸核因子-κB（0.2 倍）和白细胞介素-6（1.11 倍）的表达水平。讨论与结论 Latifolin 可通过 HIF-1α/NF-κB/IL-6 信号通路改善心肌炎症，从而预防心肌梗塞。因此，latifolin 可能是一种有前途的药物治疗缺血性心血管疾病的药物。