Acute hepatopancreatic necrosis disease (AHPND), caused by Vibrio parahaemolyticus (Vp _AHPND), is a lethal disease to Penaeus vannamei shrimp. To determine the pathogenicity of Vp _AHPND to giant river prawn (Macrobrachium rosenbergii), three immersion challenges were performed at a range of salinities. In the first trial, M. rosenbergii were challenged to observe the extent of AHPND infection. The second trial had P. vannamei exposed to Vp _AHPND at low salinities to observe the effect. Results showed the Vp _AHPND caused infection in P. vannamei at 5–20 ppt with survival rates ranging from 0% to 65%. The third trial simultaneously exposed M. rosenbergii and P. vannamei to Vp _AHPND to determine the effect of AHPND in two species while in identical environments. In trials I and III, M. rosenbergii did not show any mortality and no pathology indicative of AHPND were noted. In challenges II and III, P. vannamei exhibited 0% to 65% survival and were AHPND positive by histology and PCR. These findings indicate that M. rosenbergii held in salinities of 20 ppt and lower are not susceptible to AHPND at a dose of 1x10⁶ CFU/ml, which was lethal to P. vannamei at all salinities tested.

中文翻译：

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不同盐度对淡水虾罗氏沼虾和南美白对虾对虾的急性肝胰腺坏死病（AHPND）的致病性

急性肝胰腺坏死病（AHPND），由副溶血性弧菌（VP _AHPND），是一种致命疾病南美白对虾虾。为了确定Vp _AHPND对大河虾（Macrobrachium rosenbergii）的致病性，在一定范围的盐度下进行了3次浸没挑战。在第一个试验中，罗氏沼虾被挑战以观察AHPND感染的程度。第二项试验是将南美白对虾在低盐度下暴露于Vp _AHPND以观察其效果。结果显示Vp _AHPND引起了感染南美白对虾的生存率为5-20ppt，存活率从0％到65％不等。第三试验同时暴露罗氏沼虾和南美白对虾到VP _AHPND以确定在两个物种，而在相同的环境AHPND的效果。在试验I和III中，罗氏沼虾未显示任何死亡率，也未观察到提示AHPND的病理。在挑战II和III中，南美白对虾的存活率为0％至65％，并且通过组织学和PCR检测为AHPND阳性。这些发现表明，盐度为20 ppt或更低的罗氏沼虾对1x10 ⁶ CFU / ml的剂量的AHPND不敏感，这对所有盐度下的南美白对虾。