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Effect of the polyphenol‐rich extract from Allium cepa on hyperlipidemic sprague‐dawley rats
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-11-20 , DOI: 10.1111/jfbc.13565 Wenyi Li 1 , Chunyan Yang 1 , Xue Mei 1 , Rong Huang 1 , Shipeng Zhang 1 , Yuanyuan Tang 1 , Qiuhong Dong 1 , Chunyang Zhou 1
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-11-20 , DOI: 10.1111/jfbc.13565 Wenyi Li 1 , Chunyan Yang 1 , Xue Mei 1 , Rong Huang 1 , Shipeng Zhang 1 , Yuanyuan Tang 1 , Qiuhong Dong 1 , Chunyang Zhou 1
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Allium cepa is used for the prevention and treatment of hyperlipidemia‐related diseases such as atherosclerosis in the folk. This study was mainly aimed at investigating the effects of A. cepa extract (ACE) enriched in polyphenols on hyperlipidemia Sprague‐Dawley (SD) experiment rat models. The levels of total cholesterol (TC), triglyceride (TG), low‐density lipoprotein cholesterol (LDL‐C), high‐density lipoprotein cholesterol (HDL‐C), malondialdehyde (MDA), and superoxide dismutase (SOD) activity in serum and liver were measured using ELISA kits. In addition, hematoxylin‐eosin (HE) technique was used to observe the liver and the aortic arch pathology. Moreover, western blotting (WB) method was applied to analyze LDL receptor (LDLR) and 3‐hydroxy‐3‐methylglutaryl (HMG)‐CoA reductase (HMGCR) in liver. As a result, quercetin (2.42 mg/g DW) and isoquercitrin (4.60 mg/g DW) were the main constituents of ACE using HPLC analysis. Furthermore, ACE reduced the levels of TC, TG, LDL‐C, and MDA, and increased HDL levels and elevated SOD activity both in serum and liver in hyperlipidemic SD rats (p < .05). HE results showed that liver fat drops of the rats in ACE group were obviously decreased, and the lipid and foam cells of the aortic arch of the rats in ACE group were markedly ameliorated. WB results showed that ACE promoted the degradation of HMGCR and increased LDLR expression in liver (p < .05). In conclusion, ACE alleviated hyperlipidemia with downregulation of HMGCR and upregulation of LDLR.
中文翻译：
洋葱皮中富含多酚的提取物对高脂血症鼠瘟的影响
洋葱切皮用于预防和治疗与高脂血症相关的疾病，例如民间动脉粥样硬化。这项研究的主要目的是调查洋葱曲霉的影响高脂血症Sprague-Dawley（SD）实验大鼠模型上富含多酚的提取物（ACE）。血清中总胆固醇（TC），甘油三酸酯（TG），低密度脂蛋白胆固醇（LDL‐C），高密度脂蛋白胆固醇（HDL‐C），丙二醛（MDA）和超氧化物歧化酶（SOD）活性的水平使用ELISA试剂盒测量肝脏和肝脏。此外，苏木精曙红（HE）技术还用于观察肝脏和主动脉弓的病理情况。此外，采用Western blotting（WB）方法分析肝脏中的LDL受体（LDLR）和3-羟基-3-甲基戊二酰（HMG）-CoA还原酶（HMGCR）。结果，使用HPLC分析，槲皮素（2.42 mg / g DW）和异槲皮苷（4.60 mg / g DW）是ACE的主要成分。此外，ACE降低了TC，TG，LDL-C和MDA的水平，p <.05）。HE结果表明，ACE组大鼠肝脂肪滴明显减少，ACE组大鼠主动脉弓脂质和泡沫细胞明显改善。WB结果表明，ACE促进了HMGCR的降解并增加了肝脏中LDLR的表达（p <.05）。总之，ACE通过下调HMGCR和上调LDLR减轻高脂血症。
更新日期：2021-01-19
中文翻译：
洋葱皮中富含多酚的提取物对高脂血症鼠瘟的影响
洋葱切皮用于预防和治疗与高脂血症相关的疾病，例如民间动脉粥样硬化。这项研究的主要目的是调查洋葱曲霉的影响高脂血症Sprague-Dawley（SD）实验大鼠模型上富含多酚的提取物（ACE）。血清中总胆固醇（TC），甘油三酸酯（TG），低密度脂蛋白胆固醇（LDL‐C），高密度脂蛋白胆固醇（HDL‐C），丙二醛（MDA）和超氧化物歧化酶（SOD）活性的水平使用ELISA试剂盒测量肝脏和肝脏。此外，苏木精曙红（HE）技术还用于观察肝脏和主动脉弓的病理情况。此外，采用Western blotting（WB）方法分析肝脏中的LDL受体（LDLR）和3-羟基-3-甲基戊二酰（HMG）-CoA还原酶（HMGCR）。结果，使用HPLC分析，槲皮素（2.42 mg / g DW）和异槲皮苷（4.60 mg / g DW）是ACE的主要成分。此外，ACE降低了TC，TG，LDL-C和MDA的水平，p <.05）。HE结果表明，ACE组大鼠肝脂肪滴明显减少，ACE组大鼠主动脉弓脂质和泡沫细胞明显改善。WB结果表明，ACE促进了HMGCR的降解并增加了肝脏中LDLR的表达（p <.05）。总之，ACE通过下调HMGCR和上调LDLR减轻高脂血症。
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