Microalgae single-cell electrochemical characteristics are playing an important key role in its industrial application and functionality. This study conducted a single-cell electrochemical study to explain the influence mechanism of intracellular phenolic and flavonoid contents of microalgae on its surface electric charge. In the method, four microalgae species, Arthrospira platensis (AP), Chlorella vulgaris (CV), Phaeodactylum tricornutum (PT), and Scenedesmus obliquus (SO), were studied through single-cell electrochemical current (SCC), single-cell micro-Raman spectrometry (SCMR), and other physicochemical methods. Also, they were correlated with the chemical analytical methods. In the results, PT and AP phenolics, flavonoids, and antioxidant activities were significantly high compared to CV and SO. On the other hand, SO had the highest SCC compared to AP and PT. Also, SCMR at 1514 cm^-1 was negatively correlated with antioxidant activity by nitric oxide (r² = -0.80). In conclusion, single-cell current measurement by gold microelectrode can verify the antioxidant activity of the different microalgae species. Besides, this work provides a simple micro-level study of the single-cell phytochemicals to better explain their exact functional impacts compared to the methods of macro-analyses that use millions of cells, which reduces their sensitivity. In which, it can be applied in the real-time functionality evaluation during microalgae cultivation.

微藻单细胞电化学特性在其工业应用和功能中起着重要的关键作用。这项研究进行了单细胞电化学研究，以解释微藻的细胞内酚和类黄酮含量对其表面电荷的影响机理。在该方法中，四种微藻物种分别是：节肢动物（Arthrospira platensis）（AP），小球藻（CV），角皮氏菌（Phaeodactylum tricornutum）（PT）和斜生藻（Scenedesmus obliquus）（SO）是通过单细胞电化学电流（SCC），单细胞微拉曼光谱法（SCMR）和其他物理化学方法进行研究的。而且，它们与化学分析方法相关。结果，与CV和SO相比，PT和AP的酚类，类黄酮和抗氧化剂活性显着较高。另一方面，与AP和PT相比，SO具有最高的SCC。同样，在1514 cm ^-1处的SCMR与一氧化氮的抗氧化活性呈负相关（r ²= -0.80）。总之，通过金微电极测量单细胞电流可以验证不同微藻物种的抗氧化活性。此外，与使用数百万个细胞的宏观分析方法相比，这项工作提供了单细胞植物化学物质的简单微观水平研究，以更好地解释其确切的功能影响，从而降低了其敏感性。其中，它可以应用于微藻培养过程中的实时功能评估。