Artemisinin extracted from Artemisia annua L. plants has a range of properties that qualifies it to treat several diseases, such as malaria and cancer. However, it has short half-life, which requires making continuous use of it, which has motivated the association of artemisinin (ART) with polymeric nanoparticles to increase its therapeutic efficiency. However, the ecotoxicological safety of this association has been questioned, given the scarcity of studies in this area. Thus, in this work the toxicity of Poly (ε-Caprolactone) nanocapsules added with ART (ART-NANO) in zebrafish (Danio rerio), embryos and adults was studied. Different endpoints were analyzed in organisms exposed to ART-NANO, including those predictive of embryotoxicity and histopatoxicity. Embryotoxicity was analyzed based on Organization for Economic Co-operation and Development (OECD) test guideline (236) for fish embryo acute toxicity applied to zebrafish (Danio rerio) at 96 hpf under five nominal logarithmic concentrations (0.125 to 2.0mg L^-1). Our results demonstrate, mainly, that fertilized eggs presented increased coagulation, lack of heart rate, vitelline sac displacement and lack of somite formation. On the other hand, adult individuals (exposed to the same concentrations and evaluated after 24 and 96 h of exposure) have shown increased pericarditis. Therefore, the treatment based on ART, poly (ε-caprolactone) nanocapsules and on their combination at different concentrations have shown toxic effects on zebrafish embryos and adult individuals.

从青蒿植物中提取的青蒿素具有一系列特性，使其有资格治疗多种疾病，例如疟疾和癌症。但是，它的半衰期短，需要连续使用，这促使了青蒿素（ART）与聚合物纳米颗粒的结合，从而提高了治疗效率。然而，鉴于该领域研究的匮乏，该协会的生态毒理学安全性受到质疑。因此，在这项工作中，加入了ART（ART-NANO）的聚（ε-己内酯）纳米胶囊在斑马鱼中的毒性（Danio rerio），胚胎和成虫进行了研究。在暴露于ART-NANO的生物中分析了不同的终点，包括那些预测胚胎毒性和组织毒性的终点。根据经济合作与发展组织（OECD）测试准则（236）在五个标称对数浓度（0.125至2.0mg L ^-1）下以96hpf对斑马鱼（Danio rerio）施用鱼胚急性毒性进行了分析，分析了胚胎毒性。）。我们的研究结果主要表明，受精卵的凝结增加，心律不齐，卵黄囊移位和缺乏体节的形成。另一方面，成年个体（暴露于相同浓度并在暴露24和96小时后进行评估）显示心包炎增加。因此，基于ART，聚（ε-己内酯）纳米胶囊及其不同浓度组合的治疗已显示出对斑马鱼胚胎和成年个体的毒性作用。