Chlorothalonil (CHL) and procymidone (PRO) are fungicides that exhibit low toxicity and are widely used in many countries. And both fungicides are frequently detected in the food chain. However, the health risk posed by these fungicides is still unclear. Here, 8-week-old male C57BL/6 mice were orally treated with CHL (10, 50 mg/kg/day), PRO (20, 100 mg/kg/day) and CHL+PRO (5+10, 25+50 mg/kg/day) by dietary supplementation for 10 weeks. Hepatic pathological analysis showed that exposure to CHL, PRO and CHL+PRO could cause liver injury. The glucose, triglyceride (TG) levels and the related gene expression to glucolipid metabolism changed significantly. The significantly reduced acylcarnitine levels demonstrated that CHL, PRO and CHL+PRO exposure inhibited fatty acids (FAs) β-oxidation. In addition, CHL and PRO altered the structure of the gut microbiota and destroyed the integrity of the intestinal barrier function. In particular, AF12, Odoribacter, Prevotella and Lactobacillus were highly correlated with carnitine. The results showed that CHL, PRO and CHL+PRO exposure might inhibit FAs β-oxidation by decreasing cystic fibrosis transmembrane conductance regulator (CFTR)-mediated ion transport, indicating that these fungicides disturbed intestinal barrier function associated with glucolipid metabolism disorder. Here, the data also indicated that there was an additive effect between CHL and PRO in mice.

氯噻酮（CHL）和嘧啶酮（PRO）是杀真菌剂，具有低毒性，在许多国家被广泛使用。而且两种杀菌剂在食物链中都经常被发现。但是，这些杀菌剂对健康的危害尚不清楚。在这里，对8周大的雄性C57BL / 6小鼠口服CHL（10，50 mg / kg / day），PRO（20，100 mg / kg / day）和CHL + PRO（5 + 10，25+ 50 mg / kg /天），通过饮食补充10周。肝病理分析表明，暴露于CHL，PRO和CHL + PRO可能导致肝损伤。葡萄糖，甘油三酸酯（TG）水平和与糖脂代谢相关的基因表达发生了显着变化。酰基肉碱水平的显着降低表明CHL，PRO和CHL + PRO暴露抑制了脂肪酸（FAs）β-氧化。此外，CHL和PRO改变了肠道菌群的结构，破坏了肠屏障功能的完整性。尤其是，AF12，敖德罗杆菌，普氏杆菌和乳杆菌与肉碱高度相关。结果表明，CHL，PRO和CHL + PRO暴露可能通过减少囊性纤维化跨膜电导调节剂（CFTR）介导的离子转运而抑制FAsβ氧化，表明这些杀菌剂扰乱了与糖脂代谢异常相关的肠道屏障功能。在此，数据还表明CHL和PRO之间在小鼠中具有累加作用。