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Structural features and antioxidant activity of a new galactoglucan from edible mushroom Pleurotus djamor
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2020-11-21 , DOI: 10.1016/j.ijbiomac.2020.11.131
Gajendra Nath Maity , Prasenjit Maity , Somanjana Khatua , Krishnendu Acharya , Sudipta Dalai , Soumitra Mondal

A new water soluble galactoglucan with apparent molecular weight ~1.61 × 105 Da, was isolated from the edible mushroom Pleurotus djamor by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography. The sugar analysis showed the presence of glucose and galactose in a molar ratio of nearly 3:1 respectively. The structure of the repeating unit in the polysaccharide was determined through chemical and NMR experiments as:

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In vitro antioxidant studies showed that the PDPS exhibited hydroxyl radical scavenging activity (EC50 = 1.681 ± 0.034 mg/ml), DPPH radical scavenging activity (EC50 = 3.83 ± 0.427 mg/ml), reducing power (EC50 = 4.258 ± 0.095 mg/ml), and ABTS radical quenching activity (EC50 = 0.816 ± 0.077 mg/ml). So, PDPS should be explored as a natural antioxidant.



中文翻译:

食用菌侧耳菇新半乳糖葡聚糖的结构特征和抗氧化活性

通过热水萃取 从食用菌平菇中分离出一种新的表观分子量约为1.61×10 5 Da的水溶性半乳糖葡聚糖,然后通过透析管纤维素膜和琼脂糖6B柱色谱法进行纯化。糖分析显示葡萄糖和半乳糖分别以近3:1的摩尔比存在。通过化学和NMR实验确定多糖中重复单元的结构为:

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体外抗氧化剂研究表明,PDPS具有清除羟基自由基的活性(EC 50  = 1.681±0.034 mg / ml),具有DPPH自由基清除活性(EC 50  = 3.83±0.427 mg / ml),还原能力(EC 50  = 4.258±0.095)毫克/毫升)和ABTS自由基淬灭活性(EC 50  = 0.816±0.077毫克/毫升)。因此,PDPS应该被视为一种天然抗氧化剂。

更新日期:2020-11-22
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