Probiotic supplements are being used to improve the growth and immune performance of aquaculture species over the last couple of decades. In recent times, black soldier fly (BSF) is considered as one of the promising sources of alternative protein to fishmeal protein in aqua-diets. Since the freshwater crayfish, marron (Cherax cainii), a Western Australian's native and iconic freshwater crayfish species, grows fairly slow under commercial farming environment, this study was aimed to investigate the supplemental effect of BSF and BSF with probiotic bacteria Lactobacillus plantarum (BSFLP) on overall health and immune performance of marron after 56 days of feeding under laboratory conditions. The post-trial data revealed insignificant influences of any diets on growth performance, however, both BSF and BSFLP based diets significantly improved some haemolymph parameters and gut health of marron. High throughput sequence data revealed that both BSF and BSFLP diets significantly improved the diversity of microbial communities including some beneficial bacteria for crustaceans in the hindgut of marron. Further analysis showed that both BSF and BSFLP diets upregulated the expression of some genes in the gut tissue and haemocytes associated with the innate immune response of marron at 48 h post injection. The up-regulation of some immune genes in BSFLP diet group was found significantly linked to OTU abundance for Lactobacillus. The findings of this study could be helpful for improving overall health status of marron.

在过去的几十年中，益生菌补充剂被用于改善水产养殖物种的生长和免疫性能。近年来，黑士兵蝇（BSF）被认为是水产饮食中鱼粉蛋白质替代蛋白质的有前途的来源之一。由于淡水小龙虾马龙（Cerax cainii）是西澳大利亚州的本地和标志性淡水小龙虾，在商业化养殖环境下生长相当缓慢，因此本研究旨在研究BSF和BSF对益生菌细菌植物乳杆菌的补充作用。（BSFLP）在实验室条件下喂养56天后对马龙的整体健康和免疫性能的影响。试验后的数据显示，任何饮食对生长性能的影响均不显着，但是，基于BSF和BSFLP的饮食均显着改善了马龙的某些血淋巴参数和肠道健康。高通量序列数据表明，BSF和BSFLP饮食均显着改善了微生物群落的多样性，包括对马龙后肠中甲壳类动物有益的细菌。进一步的分析表明，BSF和BSFLP日粮在注射后48小时都上调了肠道组织和血细胞中某些基因的表达，这些基因与马龙的先天免疫反应有关。发现BSFLP饮食组某些免疫基因的上调与OTU的丰度显着相关。乳杆菌。这项研究的发现可能有助于改善马龙的整体健康状况。