Heat-killed Vibrio alginolyticus (HVa), formalin-inactivated V. alginolyticus (FVa), heat-killed Vibrio harveyi (HVh), formalin-inactivated V. harveyi (FVh), live V. alginolyticus (LVa), and live V. harveyi (LVh) were used in this study. White shrimp Litopenaeus vannamei receiving two mixtures (HVa + FVa) or four mixtures (HVa + FVa + HVh + FVh) served as primary exposure, and shrimp receiving LVa or LVh afterward served as secondary exposure. Shrimp receiving marine saline and then receiving either LVa or LVh served as controls. Phagocytic activity and clearance efficiency were examined in shrimp that received two mixtures after 1–8 weeks and then received LVa. Both the phagocytic activity and clearance efficiency of shrimp receiving two mixtures were significantly higher than in control shrimp after 1–8 weeks. In another experiment, phagocytic activity and clearance efficiency were examined in shrimp that received four mixtures after 1–8 weeks and then received LVa and LVh, respectively. The phagocytic activity of shrimp receiving four mixtures was significantly higher than in control shrimp after 1–8 weeks post exposure to LVa and LVh. The clearance efficiency of shrimp receiving four mixtures was significantly higher than in control shrimp after 1–6 weeks post exposure to LVa, and 1–7 weeks post exposure to LVh. In the other experiment, the survival rate of shrimp that received four mixtures after five weeks were challenged with LVa at 6.4 × 10⁷ colony-forming units (cfu) shrimp⁻¹ and LVh at 4.4 × 10⁶ cfu shrimp⁻¹. Shrimp that received marine saline for five weeks and then challenged with LVa and LVh at a same dose served as challenged controls. The survival rate of shrimp that received four mixtures was significantly higher (90%) than that of control shrimp (67%), and significantly higher (73%) than that of control shrimp (53%) after 3–7 days post challenge with LVa and LVh. It is concluded that the mixtures have feature of adjuvant and antigen, and shrimp receiving mixtures of heat-killed and formalin-inactivated V. alginolyticus and V. harveyi even after 5–8 weeks exhibit memory recall and show increased phagocytosis and resistance to Vibrio infections.

热灭活溶藻弧菌（HVA），福尔马林灭活溶藻弧菌（的FVa），热灭活哈维氏弧菌（HVH），福尔马林灭活哈维氏弧菌（FVH），活V。这项研究使用了溶藻藻（LVa）和活哈氏弧菌（LVh）。白对虾凡纳滨对虾接受两种混合物（HVa + FVa）或四种混合物（HVa + FVa + HVh + FVh）作为主要暴露，随后接受LVa或LVh的虾作为次要暴露。虾接受海水盐水，然后接受LVa或LVh作为对照。检查了虾的吞噬活性和清除效率，这些虾在1-8周后接受了两种混合物，然后接受了LVa。1-8周后，接受两种混合物的虾的吞噬活性和清除效率均显着高于对照虾。在另一项实验中，检查了虾的吞噬活性和清除效率，该虾在1-8周后接受了四种混合物，然后分别接受了LVa和LVh。暴露于LVa和LVh后1-8周后，接受四种混合物的虾的吞噬活性显着高于对照虾。接受四种混合物的虾的清除效率在暴露于LVa后1至6周和暴露于LVh后1至7周显着高于对照虾。在另一个实验中，用LVa以6.4×10攻击五周后接受四种混合物的虾的成活率。^7个 菌落形成单位（cfu）虾^-1和LVh（4.4×10 ⁶  cfu虾^-1）。虾接受海水盐水五周，然后用相同剂量的LVa和LVh攻击，作为攻击的对照。攻击后3-7天，接受四种混合物的虾的存活率显着高于对照虾（67％），显着高于对照虾（53％）（73％）。 LVa和LVh。结论是该混合物具有佐剂和抗原的特征，虾接受热灭活和福尔马林灭活的溶藻弧菌和哈维弧菌的混合物即使在5至8周后，仍会出现记忆记忆，并显示吞噬作用和对弧菌感染的抵抗力增强。