Analysis of particulate matter originating from beef cattle feed yards on the High Plains of the United States has revealed occurrence of multiple pesticides believed to potentially impact non-Apis pollinators. Among these pesticides are those that are highly toxic to Apis mellifera (honey bees). However, little non-Apis bee species toxicity data exist; especially pertaining to beef cattle feed yard-derived pesticides. Therefore, we conducted a series of 96-h contact toxicity tests with blue orchard mason bees (Osmia lignaria) using three neonicotinoids, two pyrethroids, and two macrocyclic lactones. Neonicotinoids (thiamethoxam, imidacloprid, and clothianidin) were most toxic with LD₅₀ values ranging from 2.88 to 26.35 ng/bee, respectively. Macrocyclic lactones (abamectin and ivermectin) were also highly toxic to O. lignaria with LD₅₀ estimates of 5.51–32.86 ng/bee. Pyrethroids (permethrin and bifenthrin) were relatively less toxic with LD₅₀ values greater than 33 ng/bee. Sensitivity ratios for each pesticide were calculated to relate O. lignaria LD₅₀ values to existing honey bee toxicity data. All three neonicotinoids were more toxic to O. lignaria than A. mellifera, but pyrethroids and abamectin were relatively less toxic. Additionally, three of seven pesticides (43%) resulted in significantly different mass normalized LD₅₀ values for male and female O. lignaria. These results indicate that non-Apis pollinators may be highly susceptible to pesticides originating from beef cattle feed yards, necessitating consideration of more stringent regulatory protections than those based on A. mellifera pesticide sensitivity.

对源自美国高平原的肉牛饲料场的颗粒物的分析表明，多种农药的发生被认为可能影响非Apis传粉者。在这些农药中，有对蜜蜂蜜蜂（Apis mellifera）（蜜蜂）有剧毒的农药。但是，几乎没有非蜜蜂的蜜蜂毒性数据。特别是关于肉牛饲料场衍生的农药。因此，我们使用三种新烟碱类化合物，两种拟除虫菊酯和两种大环内酯对蓝果园梅森蜜蜂（Osmia lignaria）进行了96小时接触毒性测试。新烟碱（噻虫嗪，吡虫啉和可比丁）对LD _50的毒性最大。值分别从2.88到26.35 ng / bee。大环内酯（阿维菌素和伊维菌素）对O. lignaria的毒性也很高，LD ₅₀估计值为5.51–32.86 ng / bee。拟除虫菊酯（苄氯菊酯和联苯菊酯）的毒性相对较低，LD ₅₀值大于33 ng /只。计算每种农药的敏感度比率，以将褐藻O. lignaria LD ₅₀值与现有蜜蜂毒性数据相关联。所有这三个新烟碱类是更具毒性O. lignaria比西方蜜蜂，但拟除虫菊酯和阿维菌素相对毒性较小。此外，七种农药中的三种（43％）导致质量归一化LD显着不同雄性和雌性O. lignaria的₅₀值。这些结果表明，非Apis传粉者可能对来自肉牛饲料场的农药高度敏感，因此有必要考虑比基于A. mellifera农药敏感性的农药更严格的法规保护。