Abstract As one of the most devastating diseases in Eriocheir sinensis, tremor disease (TD) is caused by Spiroplasma eriocheiris and dramatically increases the economic losses of crab aquaculture. With the development of biotechnology, metabolomics plays an important role in the screening of metabolites related to physiological and pathological changes. In this study, the different metabolites induced by S. eriocheiris were investigated in hemocytes of crab E. sinensis using metabolomics based on GC/MS among diverse phenotypes groups (tremor group, no-symptom group, and control group). Compared with the tremor group, 42 differential metabolites (26 up-regulated and 16 down-regulated) were significantly different in the no-symptom group. The KEGG pathway enrichment analysis showed that the differential metabolites were enriched in “ABC transporters”, “Vitamin B6 metabolism”, “Ascorbate and aldarate metabolism” and “Starch and sucrose metabolism”. The cellobiose and α-ketoglutarate (AKG) were chosen to verify the function of these two metabolites. The crabs were fed with dietary cellobiose (1, 5, and 10 mg/g diet weight) or AKG (1, 5, and 10 mg/g diet weight) before S. eriocheiris injection. The results show that all three concentrations of cellobiose and AKG could reduce the mortality of crabs and decrease the copies of S. eriocheiris in the hemocytes. The histopathological sections of the hindgut of E. sinensis show that cellobiose and AKG affect the connective tissue of the outer layer of muscle. Overall, these results provide insight into TD control and the sustainable development of aquaculture.

中文翻译：

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纤维二糖和α-酮戊二酸作为中华绒螯蟹的代谢调节剂以抵抗绒毛螺旋体感染

摘要 作为中华绒螯蟹最具破坏性的疾病之一，震颤病（TD）是由绒毛螺旋体引起的，极大地增加了蟹类养殖的经济损失。随着生物技术的发展，代谢组学在筛选与生理和病理变化相关的代谢物方面发挥着重要作用。在本研究中，使用基于 GC/MS 的代谢组学方法在不同表型组（震颤组、无症状组和对照组）中研究了毛手蟹血细胞中由毛手蟹引起的不同代谢物。与震颤组相比，无症状组有 42 种差异代谢物（26 种上调和 16 种下调）显着不同。KEGG通路富集分析显示差异代谢物富集在“ABC转运蛋白”、“维生素B6代谢”、“抗坏血酸和醛糖酸盐代谢”和“淀粉和蔗糖代谢”。选择纤维二糖和 α-酮戊二酸 (AKG) 来验证这两种代谢物的功能。螃蟹在注射 S. eriocheiris 之前用膳食纤维二糖（1、5 和 10 毫克/克饮食重量）或 AKG（1、5 和 10 毫克/克饮食重量）喂养。结果表明，三种浓度的纤维二糖和AKG均能降低螃蟹的死亡率，并减少血球中毛手鼠的拷贝数。E. sinensis 后肠的组织病理学切片显示纤维二糖和 AKG 影响肌肉外层的结缔组织。全面的，