• CaCO₃-assisted double emulsion method was developed to prepare pH-responsive nanoparticles enabling effective encapsulation of diverse molecules.

• The introducing of CaCO₃ could enable deep intratumoral penetration of therapeutic agents and effective neutralization of acidic tumor pH to favor the reverse of immunosuppressive tumor microenvironment (TME).

• The reverse of immunosuppressive TME ascribing to NLG919-mediated IDO1 inhibition and CaCO₃-mediated neutralization of acidic TME could collectively contribute to augmented chemo-immunotherapy of cancer.

中文翻译：

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CaCO 3 辅助制备用于增强化学免疫疗法的pH响应性免疫调节纳米颗粒

• 开发了CaCO ₃辅助双乳液法来制备 pH 响应纳米粒子，从而能够有效封装不同的分子。

• _{CaCO 3}的引入可以使治疗剂深入肿瘤内渗透并有效中和酸性肿瘤pH，从而有利于免疫抑制性肿瘤微环境（TME）的逆转。

• 归因于 NLG919 介导的 IDO1 抑制和 CaCO ₃介导的酸性 TME 中和的免疫抑制 TME 的逆转可能共同有助于增强癌症的化学免疫疗法。