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Effect of different muscle contraction mode on the expression of Myostatin, IGF-1, and PGC-1 alpha family members in human Vastus Lateralis muscle
Molecular Biology Reports ( IF 2.6 ) Pub Date : 2020-11-22 , DOI: 10.1007/s11033-020-06017-x
Pejman Taghibeikzadehbadr , Sadegh Shirian , Mostafa Sabouri

Muscle contraction stimulates a transient change of myogenic factors, partly related to the mode of contractions. Here, we assessed the response of IGF-1Ea, IGF-1Eb, IGF-1Ec, PGC1α-1, PGC1α-4, and myostatin to the eccentric Vs. the concentric contraction in human skeletal muscle. Ten healthy males were performed an acute eccentric and concentric exercise bout (n = 5 per group). For each contraction type, participants performed 12 sets of 10 repetitions knee extension by the dominant leg. Baseline and post-exercise muscle biopsy were taken 4 weeks before and immediately after experimental sessions from Vastus Lateralis muscle. Genes expression was measured by real-time PCR technique. There was a significant increase in PGC1α-1, PGC1α-4, IGF-1Ea and, IGF-1Eb mRNA after concentric contraction (p ≤ 0.05), while the PGC1α-4 and IGF-1Ec significantly increased after eccentric contraction (p ≤ 0.05). It is intriguing to highlight that; no significant differences between groups were evident for changes in any variables following exercise bouts (p ≥ 0.05). Our results found that concentric and eccentric contractions presented different responses in PGC1α-1, IGF-1Ea, IGF-1Eb, and IGF-1Ec mRNA. However, a similar significant increase in mRNA content was observed in PGC1α-4. Further, no apparent differences could be found between the response of genes to eccentric and concentric contraction.



中文翻译:

不同肌肉收缩方式对人侧外侧肌Myostatin,IGF-1和PGC-1 alpha家族成员表达的影响

肌肉收缩会刺激肌源性因子的短暂变化,部分与收缩方式有关。在这里,我们评估的回应IGF - 1EAIGF - 1EB,IGF - 1EC,PGC1α -1,PGC1α -4和肌肉生长抑制素偏心比。人骨骼肌的同心收缩。十名健康男性接受了一次急性离心和同心运动(n =每组5个)。对于每种收缩类型,参与者通过优势腿进行12组,每组10次重复膝盖伸展。在进行实验的前4周和后4周,分别从Vastus Lateralis肌肉进行基线和运动后肌肉活检。通过实时PCR技术测量基因表达。有在显著增加PGC1α -1,PGC1α -4,IGF - 1EA和,IGF - 1EB mRNA的同心收缩(后p  ≤0.05),而PGC1α -4和IGF - 1EC显著离心收缩后增加(p ≤0.05)。突出这一点很有趣;组之间没有差异显著为以下锻炼较量(改变任何变量是明显的p  ≥0.05)。我们的研究结果发现,同心和离心收缩中呈现出不同的反应PGC1α -1,IGF - 1EAIGF - 1EB,IGF - 1EC表达。但是,在PGC1α-4中观察到了类似的mRNA含量显着增加。此外,在基因对离心收缩和同心收缩的反应之间未发现明显差异。

更新日期:2020-11-22
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