Mammalian Genome ( IF 2.7 ) Pub Date : 2020-11-22 , DOI: 10.1007/s00335-020-09852-2 Jia Liu 1 , Xi-Chuan Deng 1 , Xiao-Yu Li 2 , Zhi-Bang Yang 1 , Guang-Yuan Zhang 1 , Ting-Ting Chen 1, 3
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Abstract
Antibiotics contribute a lot to human beings and can kill bacteria effectively. However, more and more studies show that antibiotics can disturb the intestinal microbial community. It has been widely reported that oral antibiotics can reduce the diversity of intestinal microflora, but the effect of intramuscular injection on intestinal microflora is less studied. In this study, we sequenced the intestinal microflora of mice treated with tetracycline by 16SrRNA method, and found that intramuscular injection of tetracycline (TET) can also reduce the intestinal microbial richness of mice. In addition, the results showed that within a certain range (3 mg), with the increase of TET injection concentration, the wind of intestinal microflora in mice decreased significantly. When the injection concentration reached saturation, although the amount of TET injection was increased, the degree of intestinal flora affected was not increased. The results showed that the degree of diversity decrease was in direct proportion to the amount of tetracycline injection in the saturated concentration, but not positively related to the high amount of TET injection after exceeding the saturated concentration.
Graphic Abstract
中文翻译：
肌肉注射四环素降低小鼠肠道微生物多样性
摘要
抗生素对人类贡献很大，能有效杀灭细菌。然而，越来越多的研究表明，抗生素会干扰肠道微生物群落。口服抗生素可降低肠道菌群多样性已被广泛报道，但肌肉注射对肠道菌群的影响研究较少。本研究通过16SrRNA法对四环素治疗小鼠肠道菌群进行测序，发现肌肉注射四环素（TET）也能降低小鼠肠道微生物丰富度。此外，结果表明，在一定范围内（3 mg），随着TET注射浓度的增加，小鼠肠道菌群的风向显着减少。当注入浓度达到饱和时，虽然增加了TET注射量，但对肠道菌群的影响程度并未增加。结果表明，多样性降低程度与饱和浓度下四环素注射量成正比，但与超过饱和浓度后高TET注射量不呈正相关。
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