The effect of concurrent attendance of two inhibitors of bone degradation, namely Alendronate (Ald) sodium trihydrate and Strontium (Sr), on Calcium Phosphate Cement (CPC) characteristics was explored. To this aim, 5 wt% Strontium and 21 mM Alendronate sodium trihydrate were used in calcium phosphate cement and setting time, ion and drug release were analyzed. RAW264.7 and G cell were cultured on cement samples and Tartrate-Resistant Acid Phosphatase (TRAP), Alkaline phosphatase (ALP) activity and MTT assay were studied. The results of structural analysis indicated that 21 mM Ald did not let the cement set. Therefore, colloidal silica was added to the cement formula and successfully decreased the setting time. In vitro tests showed Sr-loaded sample had a greater inhibitory effect on biocompatibility of G cells than Ald-loaded and Sr-Ald-loaded samples. In addition, the findings about osteoblast MTT and ALP activity indicated that Sr was more effective in osteogenic activity of G cells. The simultaneous presence of Ald and Sr in Calcium Phosphate Cement (CPC) was not as effective in its biocompatibility as the presence of Sr alone.

探讨了两种骨降解抑制剂（阿仑膦酸钠（Ald）三水合钠和锶（Sr））的同时存在对磷酸钙水泥（CPC）特性的影响。为此，在磷酸钙水泥中使用了5 wt％锶和21 mM阿仑膦酸钠三水合物，并分析了凝固时间，离子和药物释放。在水泥样品上培养RAW264.7和G细胞，研究了酒石酸抗性酸性磷酸酶（TRAP），碱性磷酸酶（ALP）的活性和MTT分析。结构分析结果表明，21 mM Ald不会使水泥凝固。因此，将胶体二氧化硅添加到水泥配方中并成功减少了凝结时间。体外测试表明，与Ald样品和Sr-Ald样品相比，Sr样品对G细胞的生物相容性具有更大的抑制作用。另外，关于成骨细胞MTT和ALP活性的发现表明，Sr对G细胞的成骨活性更有效。磷酸钙水泥（CPC）中同时存在Ald和Sr时，其生物相容性不如单独存在Sr时有效。