Enzymes production by solid-state cultivation in packed-bed bioreactor needs to be improved by mathematical modeling and also by experimentation. In this work, a mixture of sugarcane bagasse and wheat bran was used for the growth of the fungus Myceliophthora thermophila I-1D3b, able to secrete endoglucanase and xylanase, enzymes of interest in the second-generation ethanol production. Bench and pilot-scale bioreactors were used for the experiments, while critical parameters as bed porosity and airflow distribution were evaluated. Results showed enzymes with higher activities for the most porous medium, even though the less substrate amount to be cultivated. For the pilot-scale bioreactor, only the most porous medium was evaluated using different airflow distribution techniques. Using an inner tube for air supply resulted in more homogeneous enzyme production, with higher activities. The results here presented will be helpful for the scale-up of this class of bioreactor into industrial apparatuses.

在填充床生物反应器中固态培养的酶生产需要通过数学建模和实验来改进。在这项工作中，甘蔗渣和麦麸的混合物用于真菌Myceliophthora thermophila的生长I-1D3b，能够分泌内切葡聚糖酶和木聚糖酶，这是第二代乙醇生产中感兴趣的酶。实验中使用了台式和中试规模的生物反应器，同时评估了床孔隙率和气流分布等关键参数。结果表明酶对大多数多孔介质具有更高的活性，即使要培养的底物量较少。对于中试规模的生物反应器，使用不同的气流分布技术仅评估多孔介质。使用内管供气导致酶产生更均匀，活性更高。此处提供的结果将有助于将此类生物反应器放大为工业设备。