Background: Prostate cancer (PC) is the most common form of cancer in males and accounts for high cancer related deaths. CDCA5 may be a useful marker for predicting tumor metastasis and therapeutic target for the treatment of PC patients. In this study, we investigated the role of CDCA5 in prostate cancer progression. Immunohistochemistry was performed on 20 prostate cancer tissues. Method: We performed immunohistochemistry on 20 prostate cancer tissues. Differentially expressed gene CDCA5 in prostate cancer was screened based on TCGA database. In both DU145 and PC-3 cells, CDCA5 levels consistently affected cell proliferation, colony formation, apoptosis, migration and invasion. Result: CDCA5 knockdown significantly inhibited PC cell proliferation, migration and invasion. Furthermore, the apoptosis of DU145 and PC-3 cells was significantly increased after CDCA5 down-regulation. Further investigations revealed that CDCA5 may participate in the development of PC through interaction with TWIST1, CDH1 and CDH2. Conclusion: The present results provided a novel insight into the important and multifaceted role of CDCA5 in PC, indicating that CDCA5 is a promising biomarker and therapeutic target for PC.

中文翻译：

---

敲低CDCA5抑制细胞增殖、迁移和侵袭，并诱导前列腺癌细胞凋亡

背景：前列腺癌 (PC) 是男性中最常见的癌症形式，是癌症相关死亡人数最多的原因。CDCA5 可能是预测肿瘤转移和治疗 PC 患者的治疗靶点的有用标志物。在这项研究中，我们调查了CDCA5 在前列腺癌进展中的作用。对 20 个前列腺癌组织进行了免疫组化。方法：我们对 20 个前列腺癌组织进行了免疫组化。基于TCGA数据库筛选前列腺癌差异表达基因CDCA5。在 DU145 和 PC-3 细胞中，CDCA5 水平始终影响细胞增殖、集落形成、细胞凋亡、迁移和侵袭。结果：CDCA5敲低显着抑制PC细胞增殖、迁移和侵袭。此外，CDCA5下调后DU145和PC-3细胞凋亡显着增加。进一步的研究表明，CDCA5 可能通过与 TWIST1、CDH1 和 CDH2 的相互作用参与 PC 的发展。结论：目前的结果提供了对 CDCA5 在 PC 中的重要和多方面作用的新见解，表明 CDCA5 是 PC 的有前途的生物标志物和治疗靶点。