当前位置: X-MOL 学术Nanoscale › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
FRACTAL: Signal amplification of immunofluorescence via cyclic staining of target molecules
Nanoscale ( IF 5.8 ) Pub Date : 2020-11-13 , DOI: 10.1039/d0nr05800a
Yehlin Cho 1, 2, 3, 4 , Junyoung Seo 1, 2, 3, 4 , Yeonbo Sim 5, 6, 7, 8 , Jinkyoung Chung 4, 9, 10, 11 , Chan E. Park 1, 2, 3, 4 , Chun Gwon Park 5, 6, 7, 8, 12 , Doory Kim 4, 9, 10, 11 , Jae-Byum Chang 1, 2, 3, 4, 13
Affiliation  

In this article, we demonstrate fluorescent signal amplification via cyclic staining of target molecules (FRACTAL), a technique that can amplify the signal intensity of immunofluorescence staining more than nine-fold via simple cyclic staining of secondary antibodies. We also show that FRACTAL is compatible with four-color imaging and expansion microscopy imaging.

中文翻译:

分形:通过靶分子的循环染色放大免疫荧光的信号

在本文中,我们演示了通过靶分子的循环染色(FRACTAL)进行荧光信号放大的技术,该技术可通过对二抗进行简单的循环染色将免疫荧光染色的信号强度放大九倍以上。我们还表明,FRACTAL与四色成像和扩展显微镜成像兼容。
更新日期:2020-11-21
down
wechat
bug