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Microfluidic filter device coupled mass spectrometry for rapid bacterial antimicrobial resistance analysis
Analyst ( IF 3.6 ) Pub Date : 2020-11-9 , DOI: 10.1039/d0an01876g Dongxue Zhang 1, 2, 3, 4, 5 , Yijie Zhang 1, 2, 3, 4, 5 , Fan Yin 1, 2, 3, 4, 5 , Qin Qin 5, 6, 7, 8 , Hongyan Bi 5, 9, 10, 11 , Baohong Liu 1, 2, 3, 4, 5 , Liang Qiao 1, 2, 3, 4, 5
Analyst ( IF 3.6 ) Pub Date : 2020-11-9 , DOI: 10.1039/d0an01876g Dongxue Zhang 1, 2, 3, 4, 5 , Yijie Zhang 1, 2, 3, 4, 5 , Fan Yin 1, 2, 3, 4, 5 , Qin Qin 5, 6, 7, 8 , Hongyan Bi 5, 9, 10, 11 , Baohong Liu 1, 2, 3, 4, 5 , Liang Qiao 1, 2, 3, 4, 5
Affiliation
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The problem of antimicrobial resistance (AMR) is becoming increasingly serious. Bacteria producing extended spectrum beta-lactamase (ESBL), which can hydrolyze beta-lactam antibiotics, are among the most important drug resistant bacteria. Rapid AMR analysis methods are essential for identifying antibiotic resistant bacteria, which is of significant positive value to the clinical therapy of infectious disease. We developed a platform which integrates a sandwich microfluidic filter device with electrospray ionization mass spectrometry (ESI-MS). Bacterial cells were loaded in the sandwich microfluidic chip and antibiotic drugs were injected to pass through the blocked bacterial cells. By online ESI-MS analysis of the antibiotic drugs and their hydrolysis products, the AMR of the bacteria can be assessed within 30 minutes. Four Escherichia coli strains, namely two ESBL-positive and two ESBL-negative, were successfully discriminated using ampicillin and the third generation cephalosporin ceftriaxone. Considering the simplicity and high efficiency of the assay, the microfluidic chip integrated online ESI-MS system is promising in the rapid clinical diagnosis of ESBL-producing bacteria.
中文翻译：
微流体过滤器装置耦合质谱法用于快速细菌抗菌素耐药性分析
抗菌素耐药性（AMR）问题变得越来越严重。产生超广谱β-内酰胺酶（ESBL）的细菌可以水解β-内酰胺类抗生素，是最重要的耐药菌之一。快速的AMR分析方法对于鉴定抗药性细菌至关重要，这对传染病的临床治疗具有重要的积极价值。我们开发了一个平台，该平台将三明治式微流体过滤器设备与电喷雾电离质谱（ESI-MS）集成在一起。将细菌细胞装载到三明治微流控芯片中，并注射抗生素药物以穿过被阻断的细菌细胞。通过对抗生素药物及其水解产物进行在线ESI-MS分析，可以在30分钟内评估细菌的AMR。四个大肠杆菌菌株，即两个ESBL阳性和两个ESBL阴性，已使用氨苄西林和第三代头孢菌素头孢曲松成功鉴定。考虑到测定的简单性和高效率，集成在线ESI-MS的微流控芯片系统有望快速用于ESBL产生细菌的临床诊断。
更新日期：2020-12-09
中文翻译：
微流体过滤器装置耦合质谱法用于快速细菌抗菌素耐药性分析
抗菌素耐药性（AMR）问题变得越来越严重。产生超广谱β-内酰胺酶（ESBL）的细菌可以水解β-内酰胺类抗生素，是最重要的耐药菌之一。快速的AMR分析方法对于鉴定抗药性细菌至关重要，这对传染病的临床治疗具有重要的积极价值。我们开发了一个平台，该平台将三明治式微流体过滤器设备与电喷雾电离质谱（ESI-MS）集成在一起。将细菌细胞装载到三明治微流控芯片中，并注射抗生素药物以穿过被阻断的细菌细胞。通过对抗生素药物及其水解产物进行在线ESI-MS分析，可以在30分钟内评估细菌的AMR。四个大肠杆菌菌株，即两个ESBL阳性和两个ESBL阴性，已使用氨苄西林和第三代头孢菌素头孢曲松成功鉴定。考虑到测定的简单性和高效率，集成在线ESI-MS的微流控芯片系统有望快速用于ESBL产生细菌的临床诊断。
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