Luminous silica colloids with carbon dot incorporation for sensitive immunochromatographic assay of Zika virus,Analyst

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Luminous silica colloids with carbon dot incorporation for sensitive immunochromatographic assay of Zika virus
Analyst ( IF 3.6 ) Pub Date : 2020-11-6 , DOI: 10.1039/d0an02017f
Lai-Di Xu _{1,

2,

3,

4,

5} , Feng-Luan Du _{1,

2,

3,

4,

5} , Jin Zhu _{5,

6,

7} , Shou-Nian Ding _{1,

2,

3,

4,

5}

Affiliation

Here a novel strategy is reported of assembling silanized carbon dots (CDs) with porous silica templates to form fluorescent CD-based silica (FCS) colloids with uniformly packed CDs throughout the silica matrix. Dendritic silica spheres with highly accessible central−radial pores are adopted as a powerful absorbent host, which can form Si–O bonds with silane to directly fix the silanized CDs. The appropriate loading content of CDs on the inner surface of dendritic silica spheres is beneficial for the maximum fluorescence intensity of FCS colloids. High-quality silanized CDs endow multiple CD embedded silica spheres with excellent properties, including good fluorescence performance, excellent colloidal/optical stabilities and convenient biofunctionalization. The integration of these FCS colloids with a lateral flow strip platform provides an ultra-sensitive, specific and robust immunoassay method for the Zika NS1 protein with a visual detection limit of 10 pg mL⁻¹, and has been successfully applied to the detection of Zika virus in clinical samples. In addition, we also prepared conventional Au NP-based lateral flow test strips and applied them to the detection of Zika NS1 protein. By comparison, the detection limit of immunofluorescent CD-based silica (iFCS)-based lateral flow test strips is 100-fold lower than that of Au NP-based lateral flow strips.

中文翻译：

含碳点的夜光二氧化硅胶体用于寨卡病毒的灵敏免疫色谱分析

此处报道了一种新颖的策略，即将硅烷化碳点（CD）与多孔二氧化硅模板组装在一起，以形成荧光CD基二氧化硅（FCS）胶体，并在整个二氧化硅基质中均匀地填充CD。具有高度可访问的中心径向孔的树状二氧化硅球体被用作强大的吸收剂主体，它可以与硅烷形成Si-O键以直接固定硅烷化的CD。树突状二氧化硅球内表面上CD的适当负载量有利于FCS胶体的最大荧光强度。高质量的硅烷化CD赋予多个CD嵌入的二氧化硅球以优异的性能，包括良好的荧光性能，出色的胶体/光学稳定性和便捷的生物功能化。^-1，已成功应用于临床样品中寨卡病毒的检测。此外，我们还制备了常规的基于Au NP的侧向流动试纸，并将其用于Zika NS1蛋白的检测。相比之下，基于免疫荧光CD的二氧化硅（iFCS）的侧向流动检测条的检测限比基于Au NP的侧向流动检测条的检测限低100倍。

更新日期：2020-12-09

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