We evaluated serologic responses of cattle, bison, elk, and swine representing negative control, early vaccination (4–8 wk), late vaccination (21–28 wk) or booster vaccination, early after-experimental challenge (2–4 wk), and late after-experimental challenge (8–21 wk), in a brucellosis fluorescence polarization assay (FPA; n = 10 sera per species per treatment) using negative control sera from cattle, bison, elk, and swine (n = 5 per species). Sera from cattle shedding Brucella abortus strain RB51 in milk were also evaluated against the 20 negative control sera. The species of negative control sera used in the FPA could increase (p < 0.05) delta millipolarization (mP; delta mP = sample mP − negative control mP) results. In general, the species of negative control sera did not alter the interpretation of FPA results in control, vaccinated, or infected animals. Even after repeated RB51 vaccinations in bison, cattle, or elk, or in cattle shedding RB51 in milk, serologic results from the FPA remained negative. Species differences in FPA results were noted; elk developed robust humoral responses very quickly after infection that resulted in strong positive FPA results. In cattle and bison, humoral responses appeared to develop over a longer period of time, and greater delta mP values were detected at later times after infection. Sensitivity of the FPA for detecting infected animals was greatest for elk in early challenge samples and bison in late challenge samples. Our data suggest that species of origin of negative control sera does not influence interpretation of the FPA in natural hosts of Brucella abortus.

我们评估了牛、野牛、麋鹿和猪的血清学反应，这些反应代表阴性对照、早期疫苗接种（4-8 周）、晚期疫苗接种（21-28 周）或加强接种、试验后早期攻击（2-4 周）、和后期实验后攻击（8-21 周），在布鲁氏菌病荧光偏振测定（FPA；每次处理每个物种n = 10 个血清）中使用来自牛、野牛、麋鹿和猪的阴性对照血清（每个物种n = 5 ）。还对照 20 种阴性对照血清评估了来自牛奶中流产布鲁氏菌菌株 RB51 的牛的血清。FPA 中使用的阴性对照血清的种类可能会增加（p< 0.05) delta 毫极化（mP；delta mP = 样品 mP - 阴性对照 mP）结果。一般来说，阴性对照血清的种类不会改变对对照、接种疫苗或感染动物的 FPA 结果的解释。即使在野牛、牛或麋鹿中重复接种 RB51 疫苗，或在牛奶中脱落 RB51 的牛中，FPA 的血清学结果仍为阴性。注意到 FPA 结果中的物种差异；elk 在感染后很快产生了强烈的体液反应，导致 FPA 结果呈阳性。在牛和野牛中，体液反应似乎在更长的时间内发展，并且在感染后的后期检测到更大的 delta mP 值。FPA 检测受感染动物的敏感性对于早期攻击样本中的麋鹿和晚期攻击样本中的野牛来说是最大的。流产布鲁氏菌。