The biosynthesis of heparan sulfate (HS) proteoglycans occurs in the Golgi compartment of cells and will determine the sulfation pattern of HS chains, which in turn will have a large impact on the biological activity of the proteoglycans. Earlier studies in mice have demonstrated the importance of HS for embryonic development. In this review, the enzymes participating in zebrafish HS biosynthesis, along with a description of enzyme mutants available for functional studies, are presented. The consequences of the zebrafish genome duplication and maternal transcript contribution are briefly discussed as are the possibilities of CRISPR/Cas9 methodologies to use the zebrafish model system for studies of biosynthesis as well as proteoglycan biology.

硫酸乙酰肝素 (HS) 蛋白聚糖的生物合成发生在细胞的高尔基体区室中，并将决定 HS 链的硫酸化模式，这反过来将对蛋白聚糖的生物活性产生很大影响。早期的小鼠研究证明了 HS 对胚胎发育的重要性。在这篇综述中，介绍了参与斑马鱼 HS 生物合成的酶，以及可用于功能研究的酶突变体的描述。简要讨论了斑马鱼基因组重复和母体转录贡献的后果，以及 CRISPR/Cas9 方法使用斑马鱼模型系统进行生物合成和蛋白多糖生物学研究的可能性。