A simple RP-HPLC method was designed for the quantification of muscimol (5-(aminomethyl)-isoxazol-3-ol) present in five aqueous extracts of Amanita muscaria each from a different developmental stage. Results show that the maximum concentration of muscimol (1,210 mg/ml) was found in the young mushroom stage of development. Moreover, it was also found that this concentration progressively decreases as the fungus ages. The developed method is a simple but effective method for the quantification of muscimol, a widely important metabolite for the pharmaceutical industry as a possible treatment for tardive dyskinesia and Parkinson’s disease.

中文翻译：

---

建立一种用于分离和定量毒蝇伞不同发育阶段毒蕈醇的 RP-HPLC 方法

设计了一种简单的 RP-HPLC 方法，用于对来自不同发育阶段的毒蝇伞的五种水提取物中存在的毒蕈醇（5-（氨基甲基）-异恶唑-3-醇）进行定量。结果表明，在幼菇发育阶段发现了最大浓度的蕈蕈醇 (1,210 mg/ml)。此外，还发现该浓度随着真菌老化而逐渐降低。开发的方法是一种简单但有效的方法，用于量化 muscimol，这是制药行业广泛重要的代谢物，可作为迟发性运动障碍和帕金森病的一种可能治疗方法。