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Evaluating adenosine triphosphate bioluminescence for biomonitoring in potable water systems
AQUA - Water Infrastructure, Ecosystems and Society Pub Date : 2020-11-01 , DOI: 10.2166/aqua.2020.016
Angela B. Rodriguez 1 , Steven J. Duranceau 2
Affiliation  

The application of adenosine triphosphate (ATP) bioluminescence technology as a rapid microbiological activity monitor within a potable water system has been demonstrated. ATP measurements were taken pre- and post-infrastructure improvements within a watershed and throughout two potable water systems from source to tap. A reduction in ATP, as measured by relative light units, was identified post-infrastructure improvements. Peak ATP values for the watershed were found within the reservoirs. The treated source water remained biologically stable throughout the distribution system, with peaks attributed to nitrification. A mathematical model for predicting microbial ATP using pH, temperature, and alkalinity, was developed for the watershed studied, with an adjusted R2 of 0.84 at a 95% confidence level. Overall, ATP bioluminescence technology was found to be a suitable candidate for rapid microbiological activity testing in drinking water systems; however, technological limitations remain with respect to reproducibility that should be addressed prior to full-scale implementation.



中文翻译:

评估三磷酸腺苷的生物发光以用于饮用水系统中的生物监测

已经证明了三磷酸腺苷(ATP)生物发光技术在饮用水系统中作为快速微生物活性监测器的应用。ATP测量是在流域内以及整个两个饮用水系统中从源头到水龙头进行基础设施改进之前和之后进行的。通过相对光单位测得的ATP降低被确定为基础设施改善。在水库内发现了流域的ATP峰值。经过处理的水源在整个分配系统中均保持生物学稳定,其峰值归因于硝化作用。针对所研究的分水岭,开发了使用pH,温度和碱度预测微生物ATP的数学模型,并调整了R 295%置信水平下的0.84。总的来说,ATP生物发光技术被认为是饮用水系统中快速微生物活性测试的合适候选人。但是,在可重复性方面仍存在技术限制,应在全面实施之前解决。

更新日期:2020-11-21
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