Abstract

The synthesis of zinc oxide nanoparticles (ZnO-NPs) provides interesting biological characteristics and therapeutic applications in a particular method, which evolve the versatile platform for the essential development of the new anticancer agents. Cytotoxic effect of ZnO-NPs was evaluated by MTT assay on A549 cell line. Besides, the anti-apoptotic effects of ZnO-NPs were investigated using fluorescent microscopy and flow cytometry techniques. Histological assessment was carried out using H&E staining. Finally, complete blood count and hematological factors were analyzed on RBC, platelets, and WBC. Obtained data showed the slight cellular viability (IC₅₀ = 37.2 after 24 h) and increased sub-G1 cell cycle arrest of A549 lung carcinoma cells under ZnO-NPs exposure, indicate the significant dose-dependent and time-dependent cytotoxic effects. In vivo analysis demonstrated raise level of MCH, RBC, and WBC count. ZnO-NPs have the sufficient potential used to be as a safe efficient apoptotic inducer especially in lung cancer therapy.

摘要

氧化锌纳米粒子 (ZnO-NPs) 的合成在特定方法中提供了有趣的生物学特性和治疗应用，这为新抗癌剂的基本开发提供了通用平台。通过对 A549 细胞系的 MTT 测定评估 ZnO-NPs 的细胞毒性作用。此外，使用荧光显微镜和流式细胞术技术研究了 ZnO-NPs 的抗细胞凋亡作用。使用 H&E 染色进行组织学评估。最后，对 RBC、血小板和 WBC 进行全血细胞计数和血液学因素分析。获得的数据显示轻微的细胞活力（IC ₅₀= 37.2 后 24 小时）和在 ZnO-NPs 暴露下 A549 肺癌细胞的亚 G1 细胞周期停滞增加，表明显着的剂量依赖性和时间依赖性细胞毒性作用。体内分析表明 MCH、RBC 和 WBC 计数水平升高。ZnO-NPs 有足够的潜力被用作安全有效的凋亡诱导剂，尤其是在肺癌治疗中。