Abstract

The complete mitogenome of Lasioglossum affine (Hymenoptera: Halictidae) was sequenced and analyzed. The whole mitogenome is 17,352 bp (AT%=84.1%) and encodes 37 typical eukaryotic mitochondrial genes, including 13 protein-coding genes (PCGs), 22 tRNAs, two rRNAs, and an AT-rich region. Further analysis found three gene rearrangements, where trn I-Q-M → trn M-I-Q, trn W-C-Y → trn C-W-Y, and trn K-D → trn D-K were shuffled. The phylogenetic relationships of 19 species of Hymenoptera were established using maximum-likelihood method based on 13 concatenated PCGs. The result showed that Lasioglossum affine is a sister of Lasioglossum sp. SJW-2017.

摘要

Lasioglossum affine（膜翅目：Halictidae）的完整有丝分裂基因组进行了测序和分析。整个有丝分裂基因组为17352 bp（AT％= 84.1％），编码37个典型的真核线粒体基因，包括13个蛋白质编码基因（PCG），22个tRNA，2个rRNA和一个AT丰富的区域。进一步的分析发现三个基因重排，其中trn IQM  →  trn MIQ，trn WCY  →  trn CWY和trn KD  →  trn DK被改组。基于13个连锁PCG，采用最大似然法建立了19种膜翅目的系统发育关系。结果表明Lasioglossum仿射是Lasioglossum sp。的姐妹。SJW-2017。