ABSTRACT

Objectives: Near-Infrared based imaging modalities integrated with thermotherapy can facilitate detection of cancer at early stages and mediate high-resolution image-guided hyperthermia. In this work, fluorescent iron oxide nanoparticles (FIO) have been developed possessing deep tissue penetrable NIR imaging and site-specific magnetic hyperthermia characteristics for the elimination of cancer cells.

Methods: One-pot synthesis of amine-functionalized superparamagnetic iron oxide nanoparticles (HIO) were achieved using ethylenediamine (EDA) facilitated conjugation of indocyanine green (ICG) mediated by electrostatic interactions.

Results: EDA acts as a capping and reducing agent to direct the structural growth of hydrophilic Fe₃O₄ nanocrystals with high saturation magnetization, specific absorption rate, and T₂ value of 118 emu/g, 329.8 ± 5.96 W/g, and 40.17 mM⁻¹s⁻¹, respectively. Here, Fe²⁺/Fe³⁺ of two was maintained to achieve magnetite nanocrystals contradictory to the gold standard ratio of 0.5 without additives for nucleation and growth. Developed FIO showed excellent cytocompatibility even at higher concentrations and on subjecting to magnetic hyperthermia reduced its survival percentage. FIO biodistribution in mice showed enhanced half-life than free ICG with preferential localization in the brain and liver.

Conclusion: Developed FIO using a facile technique is a potential clinical alternative for cellular tracking, imaging, and hyperthermia.

摘要

目的：结合热疗法的基于近红外的成像方式可以促进早期阶段的癌症检测，并介导高分辨率的图像引导热疗。在这项工作中，已经开发出具有深层组织可穿透NIR成像和针对特定部位的磁热疗特性的荧光氧化铁纳米粒子（FIO），用于消除癌细胞。

方法：使用乙二胺（EDA）促进了通过静电相互作用介导的吲哚菁绿（ICG）的共轭反应，实现了一锅合成胺官能化的超顺磁性氧化铁纳米粒子（HIO）。

结果：EDA用作封闭剂和还原剂，指导亲水性Fe ₃ O ₄纳米晶体的结构生长，该纳米晶体具有高饱和磁化强度，比吸收率和T ₂值为118 emu / g，329.8±5.96 W / g和40.17 mM ^-1 s ^-1。在这里，Fe ²⁺ / Fe ³⁺保持2分之1以达到与0.5的金标准比（不含成核剂和生长剂）相反的磁铁矿纳米晶体。发达的FIO即使在更高的浓度下也显示出优异的细胞相容性，并且在经受磁热疗时降低了其存活率。小鼠中FIO的生物分布显示其半衰期比游离ICG延长，并且在大脑和肝脏中优先定位。

结论：使用简便的技术开发的FIO是细胞跟踪，成像和热疗的潜在临床替代方法。