Rare earth elements (REEs) are widely used across different industries due to their exceptional magnetic and electrical properties. In this work, Cupriavidus necator is characterized using dielectrophoretic ultra‐high‐frequency measurements, typically in MHz range to quantify the properties of cytoplasm in C. necator for its metal uptake/bioaccumulation capacity. Cupriavidus necator, a Gram‐negative bacteria strain is exposed to REEs like europium, samarium, and neodymium in this study. Dielectrophoretic crossover frequency experiments were performed on the native C. necator species pre‐ and post‐exposure to the REEs at MHz frequency range. The net conductivity of native C. necator, Cupriavidus europium, Cupriavidus samarium, and Cupriavidus neodymium are 15.95 ± 0.029 μS/cm, 16.15 ± 0.028 μS/cm, 16.05 ± 0.029 μS/cm, 15.61 ± 0.005 μS/cm respectively. The estimated properties of the membrane published by our group are used to develop a microfluidic sorter by modeling and simulation to separate REE absorbed C. necator from the unabsorbed native C. necator species using COMSOL Multiphysics commercial software package v5.5.

中文翻译：

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Cupriavidus necator对稀土元素摄取的介电泳超高频表征和硅片分选

稀土元素 (REE) 因其卓越的磁和电特性而被广泛应用于不同行业。在这项工作中，Cupriavidus necator使用介电泳超高频测量进行表征，通常在 MHz 范围内量化C. necator中细胞质的金属吸收/生物积累能力的特性。在本研究中，Cupriavidus necator是一种革兰氏阴性细菌菌株，暴露于铕、钐和钕等稀土元素。在 MHz 频率范围内对 REE 暴露前和暴露后的原生C. necator物种进行介电泳交叉频率实验。天然C. necator的净电导率，Cupriavidus europium、Cupriavidus samarium和Cupriavidus neodymium分别为15.95± 0.029μS /cm、16.15± 0.028μS /cm、16.05±0.029μS/cm、15.61±0.005μS/cm 我们小组发布的膜的估计特性用于通过建模和模拟开发微流体分选器，以使用 COMSOL Multiphysics 商业软件包 v5.5将 REE 吸收的C. necator与未吸收的天然C. necator物种分开。