This study aimed to examine 25OHD₃ concentration in the fluid of follicular and follicular lutein cysts of sows in comparison with preovulatory follicles as well as immunolocalize vitamin D metabolic enzymes (CYP27B1 and CYP24A1) and determine their protein abundances in the cyst wall. We have shown for the first time that 25OHD₃ level in the fluid of both cyst types was significantly lower than in preovulatory follicles. Furthermore, we have demonstrated CYP27B1 and CYP24A1 protein immunolocalization and abundance in follicular and follicular lutein cysts. The abundance of protein for both metabolic enzymes was decreased in ovarian cysts when compared to preovulatory follicles. We propose that altered VD metabolism in ovarian cyst might associate with their formation in sows.

中文翻译：

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母猪卵泡囊肿中维生素D代谢系统改变

这项研究旨在检查与排卵前卵泡以及免疫定位的维生素D代谢酶（CYP27B1和CYP24A1）相比，母猪卵泡和卵泡叶黄素囊液中25OHD _3的浓度，并确定其在囊壁中的蛋白丰度。我们首次显示两种囊肿类型的液体中25OHD _3的水平显着低于排卵前卵泡。此外，我们已经证明了CYP27B1和CYP24A1蛋白在卵泡和卵泡叶黄素囊肿中的免疫定位和丰度。与排卵前卵泡相比，卵巢囊肿中两种代谢酶的蛋白质丰度都降低了。我们建议改变卵巢囊肿中的VD代谢可能与其在母猪中的形成有关。