Identification of a newly isolated Rhodotorula mucilaginosa NQ1 and its development for the synthesis of bulky carbonyl compounds by whole‐cell bioreduction,Letters in Applied Microbiology

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Identification of a newly isolated Rhodotorula mucilaginosa NQ1 and its development for the synthesis of bulky carbonyl compounds by whole‐cell bioreduction
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-12-08 , DOI: 10.1111/lam.13431
N. Wang _{1,

2} , Y. Xu ₁ , C. Peng ₁ , X. Wang ₁ , Y. Wei ₁ , K. Li ₁ , S. Wang ₁ , A. Xu ₁ , J. Gao ₁

Affiliation

A strain NQ1, which showed efficient asymmetric reduction of 3,5-bis(trifluoromethyl) acetophenone (BTAP) to enantiopure (S)-[3,5-bis(trifluoromethyl)phenyl]ethanol ((S)-BTPE), which is the key intermediate for the synthesis of a receptor antagonist and antidepressant, was isolated from a soil sample. Based on its morphological and internal transcribed spacer sequence, the strain NQ1 was identified to be Rhodotorula mucilaginosa NQ1. Some key reaction parameters involved in the bioreduction catalyzed by whole cells of R. mucilaginosa NQ1 were subsequently optimized, and the optimized conditions for the synthesis of (S)-BTPE were determined to be as follows: 5.0 ml phosphate buffer (200 mmol l-1 , pH 7.0), 80 mmol l-1 of BTAP, 250 g (wet weight) l-1 of resting cell, 35 g l-1 of glucose, and a reaction for 18 h at 30°C and 180 rpm. The strain NQ1 exhibited a best yield of 99% and an excellent enantiomeric excess of 99% for the preparation of (S)-BTPE under the above optimal conditions, and could also asymmetrically reduce a variety of bulky prochiral carbonyl compounds to their corresponding optical hydroxyl compound with excellent enantioselectivity. These results indicated that R. mucilaginosa NQ1 had a good capacity to reduce BTAP to its corresponding (S)-BTPE, and might be a new potential biocatalyst for the production of valuable chiral hydroxyl compounds in industry.

中文翻译：

鉴定新分离的粘液红酵母 NQ1 及其通过全细胞生物还原合成大体积羰基化合物的进展

菌株 NQ1，显示 3,5-双（三氟甲基）苯乙酮（BTAP）有效不对称还原为对映体纯（S）-[3,5-双（三氟甲基）苯基]乙醇（（S）-BTPE），这是从土壤样品中分离出合成受体拮抗剂和抗抑郁药的关键中间体。根据其形态和内部转录间隔序列，菌株 NQ1 被鉴定为粘液红酵母 NQ1。随后，对粘液菌 NQ1 全细胞催化的生物还原反应中涉及的一些关键反应参数进行了优化，确定了合成 (S)-BTPE 的优化条件如下： 5.0 ml 磷酸盐缓冲液（200 mmol l- 1，pH 7.0)，80 mmol l-1 BTAP，250 g (湿重) l-1 静息细胞，35 g l-1 葡萄糖，并在 30°C 和 180 rpm 下反应 18 小时。在上述最佳条件下，菌株 NQ1 表现出 99% 的最佳产率和 99% 的优异对映体过量，用于制备 (S)-BTPE，并且还可以不对称地将各种庞大的前手性羰基化合物还原为相应的光学羟基具有优异的对映选择性的化合物。这些结果表明，R. mucilaginosa NQ1 具有将 BTAP 还原为其相应的 (S)-BTPE 的良好能力，并且可能成为工业上生产有价值的手性羟基化合物的新型潜在生物催化剂。

更新日期：2020-12-08

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