Ultrasensitive detection of Listeria monocytogenes using solid‐state electrochemiluminescence biosensing based on the quenching effect of ferrocene on ruthenium pyridine,Journal of Food Safety

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Ultrasensitive detection of Listeria monocytogenes using solid‐state electrochemiluminescence biosensing based on the quenching effect of ferrocene on ruthenium pyridine
Journal of Food Safety ( IF 1.9 ) Pub Date : 2020-11-20 , DOI: 10.1111/jfs.12868
Weifeng Chen ₁ , Jinsong Wu ₁ , Shan Li ₁ , Hefan Zhang ₁ , Liwei Cui ₁ , Juntao Liu ₁ , Wang Yao ₂

Affiliation

A solid‐state electrochemiluminescence (ECL) biosensing switch system based on the specific recognition of an aptamer and the destruction of pyridine ruthenium by ferrocene was successfully developed for the detection of Listeria monocytogenes detections. The switching system consisted of two principal parts, the ECL luminescent substrate and the ECL intensity switch. The ECL luminescent substrate was fabricated by attaching an aptamer of Listeria monocytogenes modified with DNA labeled with bipyridine ruthenium complementary to one end of the aptamer, and ferrocene‐labeled DNA complementary to the other end of the aptamer, to an electrode, and so act as an intensity switch. The identification reaction consisted of the specific binding of the aptamer with Listeria monocytogenes causing the labeled ferrocene to be removed from the luminescent substrate, the structural change resulting in a significant increase in the intensity of ECL due to the decreased quenching effect of ferrocene toward the ECL luminescent substrate. Accordingly, the change in ECL intensity indirectly reflected the concentration of Listeria monocytogenes in the sample. The results demonstrated that the system produced a good linear relationship over the concentration range of 1.4 × 10¹–1.4 × 10⁶ CFU/ml. The present study established that this was a feasible approach for the detection of Listeria monocytogenes in milk and pork samples.

中文翻译：

基于二茂铁对钌吡啶猝灭作用的固态电化学发光生物传感超灵敏检测李斯特菌

基于对适体的特异性识别和二茂铁对吡啶钌的破坏，基于固态电化学发光（ECL）的生物传感开关系统已成功开发用于检测单核细胞增生李斯特菌。开关系统由两个主要部分组成，ECL发光基板和ECL强度开关。ECL发光底物是通过将修饰有联吡啶钌标记的DNA修饰的单核细胞增生性李斯特氏菌的适体与适体的一端连接，并将二茂铁标记的DNA与适体的另一端互补而连接到电极上，从而充当强度开关。鉴定反应包括适体与单核细胞增生李斯特菌引起标记的二茂铁从发光底物上去除，由于二茂铁对ECL发光底物的淬灭作用降低，结构变化导致ECL强度显着增加。因此，ECL强度的变化间接反映了样品中单核细胞增生性李斯特菌的浓度。结果表明，该系统在1.4×10 ¹ –1.4×10 ⁶ CFU / ml的浓度范围内产生了良好的线性关系。本研究确定这是检测牛奶和猪肉样品中单核细胞增生李斯特氏菌的可行方法。

更新日期：2020-11-20

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