Expression of the core promoter factors TATA box binding protein and TATA box binding protein‐related factor 2 in Drosophila germ cells and their distinct functions in germline development,Development, Growth & Differentiation

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Expression of the core promoter factors TATA box binding protein and TATA box binding protein‐related factor 2 in Drosophila germ cells and their distinct functions in germline development
Development, Growth & Differentiation ( IF 1.7 ) Pub Date : 2020-11-20 , DOI: 10.1111/dgd.12701
Shoichi Nakamura _{1,

2,

3,

4} , Seiji Hira _{1,

2,

3} , Makoto Kojima ₁ , Akane Kondo _{1,

2} , Masanori Mukai _{1,

2,

3}

Affiliation

In Drosophila, the expression of germline genes is initiated in primordial germ cells (PGCs) and is known to be associated with germline establishment. However, the transcriptional regulation of germline genes remains elusive. Previously, we found that the BTB/POZ‐Zn‐finger protein, Mamo, is necessary for the expression of the germline gene, vasa, in PGCs. Moreover, truncated Mamo lacking the BTB/POZ domain (MamoAF) is a potent vasa activator. In this study, we investigated the genetic interaction between MamoAF and specific transcriptional regulators to gain insight into the transcriptional regulation of germline development. We identified a general transcription factor, TATA box binding protein (TBP)‐associated factor 3 (TAF3/BIP2), and a member of the TBP‐like proteins, TBP‐related factor 2 (TRF2), as new genetic modifiers of MamoAF. In contrast to TRF2, TBP was found to show no genetic interaction with MamoAF, suggesting that Trf2 has a selective function. Therefore, we focused on Trf2 expression and investigated its function in germ cells. We found that Trf2 mRNA, rather than Tbp mRNA, was preferentially expressed in PGCs during embryogenesis. Depletion of TRF2 in PGCs resulted in decreased mRNA expression of vasa. RNA interference‐mediated knockdown showed that, while Trf2 is required for maintenance of germ cells, Tbp is needed for their differentiation during oogenesis. Therefore, these results suggest that Trf2 and Tbp expression is differentially regulated in germ cells and that these factors have distinct functions in Drosophila germline development.

中文翻译：

核心启动子因子TATA盒结合蛋白和TATA盒结合蛋白相关因子2在果蝇生殖细胞中的表达及其在生殖发育中的不同功能

在果蝇中，种系基因的表达在原始生殖细胞 (PGC) 中开始，并且已知与种系建立有关。然而，生殖系基因的转录调控仍然难以捉摸。以前，我们发现 BTB/POZ-Zn-finger 蛋白 Mamo 是PGC 中生殖系基因vasa表达所必需的。此外，缺乏 BTB/POZ 结构域 (MamoAF) 的截短 Mamo 是一种有效的血管活化剂。在这项研究中，我们研究了 MamoAF 与特定转录调节因子之间的遗传相互作用，以深入了解种系发育的转录调控。我们鉴定了一种通用转录因子 TATA 盒结合蛋白 (TBP) 相关因子 3 (TAF3/BIP2) 和 TBP 样蛋白的成员 TBP 相关因子 2 (TRF2) 作为 MamoAF 的新遗传修饰物。与 TRF2 相比，发现 TBP 与 MamoAF 没有遗传相互作用，表明Trf2具有选择性功能。因此，我们专注于Trf2表达并研究其在生殖细胞中的功能。我们发现，TRF2表达，而不是TBPmRNA，在胚胎发生过程中优先在 PGC 中表达。PGC 中 TRF2 的消耗导致vasa 的mRNA 表达降低。RNA干扰介导击倒表明，虽然TRF2需要维护生殖细胞，TBP是需要卵子发生过程中的分化。因此，这些结果表明，TRF2和TBP表达在生殖细胞中差异调节的，并且这些因素都在不同的功能果蝇种系发育。

更新日期：2020-12-21

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