A novel fluorescence turn-on assay for heparin has been developed based on DNA-templated gold nanoclusters (AuNCs) and Cytochrome c (Cyt c). In the design, poly A (A15) ssDNA was selected as a template for fabricating AuNCs. AuNCs possess strong fluorescence emission at 471 nm. Upon addition of Cyt c, the fluorescence of AuNCs was quenched effectively by electron transfer (ET) between AuNCs and the heme cofactor of Cyt c. In the presence of heparin, the much higher electrostatic binding of heparin to Cyt c caused Cyt c to be away from AuNCs, leading to a recovery of fluorescence intensity of AuNCs. The proposed method was found to be simple, fast, and sensitive for heparin detection, with a linear range from 0.5 μg/mL to 12 μg/mL, and a detection limit of 0.15 μg/mL. Furthermore, this work was successfully applied to detection of heparin in fetal bovine serum samples, with good recoveries from 93.53% to 103.6%.

中文翻译：

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基于 DNA 模板化金纳米团簇的肝素开启荧光检测通过 ET

基于 DNA 模板化的金纳米团簇 (AuNCs) 和细胞色素 c (Cyt c) 开发了一种新型的肝素荧光开启检测。在设计中，poly A (A15) ssDNA 被选为制造 AuNCs 的模板。AuNCs 在 471 nm 处具有强烈的荧光发射。添加 Cyt c 后，AuNCs 和 Cyt c 的血红素辅因子之间的电子转移（ET）有效地淬灭了 AuNCs 的荧光。在肝素存在下，肝素与 Cyt c 的高得多的静电结合导致 Cyt c 远离 AuNC，导致 AuNC 的荧光强度恢复。发现该方法对肝素检测简单、快速且灵敏，线性范围为 0.5 μg/mL 至 12 μg/mL，检出限为 0.15 μg/mL。此外，