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A Hierarchical Janus Nanofibrous Membrane Combining Direct Osteogenesis and Osteoimmunomodulatory Functions for Advanced Bone Regeneration
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2020-11-20 , DOI: 10.1002/adfm.202008906 Qian Wang 1 , Yunbo Feng 1 , Min He 2 , Weifeng Zhao 1 , Li Qiu 3 , Changsheng Zhao 1
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2020-11-20 , DOI: 10.1002/adfm.202008906 Qian Wang 1 , Yunbo Feng 1 , Min He 2 , Weifeng Zhao 1 , Li Qiu 3 , Changsheng Zhao 1
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An ideal guided bone regeneration membrane (GBRM) is expected not only to perform barrier function, but also to enhance osteogenesis and resist bacteria infection. However, currently available membranes have limited bioactivities. To address this challenge, a Janus GBRM (JGM) is designed and fabricated by sequential fractional electrospinning here. The random gelatin fibers loaded with hydroxyapatite (HAP) are designed as the inner face to promote the osteoblasts’ adhesion, proliferation, and osteogenic differentiation, meanwhile the aligned poly(caprolactone) (PCL) nanofibers loaded with poly(methacryloxyethyltrimethyl ammonium chloride‐co‐2‐Aminoethyl 2‐methylacrylate hydrochloride) (P(DMC‐AMA)) are designed as the outer layer to resist epithelia invasion and bacterial infection. In vitro assays reveal that the inner face displays enhanced osteogenic effects, meanwhile the outer surface can regulate the epithelia to spread along the aligned direction and kill the contacted bacteria. Interestingly, the outer face can induce macrophages to polarize toward the M2 phenotype, thus manipulating a favorable osteoimmune environment. These results suggest that the JGM simultaneously meets the critical requirements of barrier, osteogenic, antibacterial, and osteoimmunomodulatory functions. Consequently, the JGM shows better in vivo bone tissue regeneration performance than the commercial Bio‐Gide membrane. This work provides a novel platform to design multi‐functional membranes/scaffolds, displaying great potential applications in tissue engineering.
中文翻译：
结合直接成骨和骨免疫调节功能的高级骨再生的分层Janus纳米纤维膜。
理想的引导骨再生膜（GBRM）不仅可以发挥屏障功能，还可以增强成骨作用并抵抗细菌感染。然而，当前可用的膜具有有限的生物活性。为了解决这一挑战，在这里通过顺序分数电纺设计并制造了Janus GBRM（JGM）。装载有羟基磷灰石（HAP）的随机明胶纤维被设计为内面，以促进成骨细胞粘附，增殖和成骨分化，同时对准的聚（己内酯）装入聚（methacryloxyethyltrimethyl铵氯化物（PCL）纳米纤维共-2-氨基丙烯酸2-甲基丙烯酸乙酯盐酸盐（P（DMC-AMA））被设计为可抵抗上皮细胞入侵和细菌感染的外层。体外试验显示，内表面显示出增强的成骨作用，同时外表面可调节上皮细胞沿排列方向扩散并杀死接触的细菌。有趣的是，外表面可诱导巨噬细胞向M2表型极化，从而操纵良好的骨免疫环境。这些结果表明，JGM同时满足屏障，成骨，抗菌和骨免疫调节功能的关键要求。因此，与商用Bio-Gide膜相比，JGM在体内的骨组织再生性能更好。这项工作为设计多功能膜/支架提供了一个新颖的平台，
更新日期：2020-11-20
中文翻译：
结合直接成骨和骨免疫调节功能的高级骨再生的分层Janus纳米纤维膜。
理想的引导骨再生膜（GBRM）不仅可以发挥屏障功能，还可以增强成骨作用并抵抗细菌感染。然而，当前可用的膜具有有限的生物活性。为了解决这一挑战，在这里通过顺序分数电纺设计并制造了Janus GBRM（JGM）。装载有羟基磷灰石（HAP）的随机明胶纤维被设计为内面，以促进成骨细胞粘附，增殖和成骨分化，同时对准的聚（己内酯）装入聚（methacryloxyethyltrimethyl铵氯化物（PCL）纳米纤维共-2-氨基丙烯酸2-甲基丙烯酸乙酯盐酸盐（P（DMC-AMA））被设计为可抵抗上皮细胞入侵和细菌感染的外层。体外试验显示，内表面显示出增强的成骨作用，同时外表面可调节上皮细胞沿排列方向扩散并杀死接触的细菌。有趣的是，外表面可诱导巨噬细胞向M2表型极化，从而操纵良好的骨免疫环境。这些结果表明，JGM同时满足屏障，成骨，抗菌和骨免疫调节功能的关键要求。因此，与商用Bio-Gide膜相比，JGM在体内的骨组织再生性能更好。这项工作为设计多功能膜/支架提供了一个新颖的平台，
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