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Identification of ACC synthetase genes in Volvariella volvacea and analysis of their response to ethephon and 1-methylcyclopropene treatments
Scientia Horticulturae ( IF 3.9 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.scienta.2020.109848
Juanqin Chen , Tianci Chen , Mingmeng Qiu , Ling Li , Qiangui Zhong , Qi Wei , Youjin Deng , Baogui Xie , Yuji Jiang , Bingzhi Chen

Abstract Straw mushroom (Volvariella volvacea) is economically important in China, but after harvest, it can be stored for only 1–2 d, because it rapidly deteriorates. The role of the ethylene signal transduction pathway in the regulation of these processes in higher fungi is unclear. In this study, we aimed to identify and analyze the acs genes in the genome of V. volvacea. Bioinformatic analyses were performed to identify differences between the acs sequences of V. volvacea and homolog gene sequences of plants. Furthermore, ethylene production and acs expression in V. volvacea during storage were measured before and after ethephon and 1-methylcyclopropene (1-MCP) treatments. The results showed that there are four acs genes in the genome of V. volvacea, and the phylogenetic tree revealed that the proteins encoded by these genes had high homology to the ACS proteins in plants, with some differences. A conserved motif was found in these ACS proteins. Ethephon accelerated endogenous ethylene production and upregulated acs expression in V. volvacea, whereas 1-MCP inhibited endogenous ethylene production and downregulated acs expression in V. volvacea. Our study provides valuable data to further investigate the roles of acs in the mechanisms of senescence of V. volvacea. Further, acs could be used to develop molecular markers enabling screening and selective breeding of V. volvacea strains with slowed senescence, prolonging the shelf life of the product.

中文翻译:

草履虫ACC合成酶基因的鉴定及其对乙烯利和1-甲基环丙烯处理的响应分析

摘要 草菇(Volvariella volvacea)在我国具有重要的经济价值,但采收后由于易腐烂,只能保存1-2天。乙烯信号转导途径在高等真菌中调节这些过程中的作用尚不清楚。在这项研究中,我们旨在鉴定和分析草履虫基因组中的 acs 基因。进行生物信息学分析以鉴定草履虫的 acs 序列和植物的同源基因序列之间的差异。此外,在乙烯利和 1-甲基环丙烯 (1-MCP) 处理之前和之后测量了储存期间草菇中乙烯的产生和 acs 表达。结果表明,草履虫基因组中有4个acs基因,系统发育树显示,这些基因编码的蛋白与植物中的ACS蛋白具有高度同源性,但存在一定差异。在这些 ACS 蛋白中发现了一个保守的基序。乙烯利加速了草菇内源性乙烯的产生并上调了草履虫中的 acs 表达,而 1-MCP 抑制了草菇中的内源乙烯产生并下调了 acs 表达。我们的研究为进一步研究 acs 在草履虫衰老机制中的作用提供了有价值的数据。此外,acs 可用于开发分子标记,从而能够筛选和选择性育种延缓衰老的草履虫菌株,延长产品的保质期。乙烯利加速了草菇内源性乙烯的产生并上调了草履虫中的 acs 表达,而 1-MCP 抑制了草菇中的内源乙烯产生并下调了 acs 表达。我们的研究为进一步研究 acs 在草履虫衰老机制中的作用提供了有价值的数据。此外,acs 可用于开发分子标记,从而能够筛选和选择性育种延缓衰老的草履虫菌株,延长产品的保质期。乙烯利加速了草菇内源性乙烯的产生并上调了草履虫中的 acs 表达,而 1-MCP 抑制了草菇中的内源乙烯产生并下调了 acs 表达。我们的研究为进一步研究 acs 在草履虫衰老机制中的作用提供了有价值的数据。此外,acs 可用于开发分子标记,从而能够筛选和选择性育种延缓衰老的草履虫菌株,延长产品的保质期。
更新日期:2021-02-01
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