Abstract Nutrition-sensitive agriculture is a novel concept in the agri-food system, which considers the implementation of techniques able to guarantee the nutritional value of the produce, the sustainability of the production and, at the same time, to reduce the ecological impact of agricultural practices. These principles can be also introduced in the fresh-cut market with the aim of maintaining the produce quality during shelf life. In this context, the use of bio-based products is rapidly increasing for improving economic and environmental sustainability of cropping systems during cultivation and shelf life. The aim of this work was to evaluate the effects of three different bacterial-based formulations (Paenibacillus pasadenensis, Bacillus amyloliquefaciens, Pseudomonas syringae) applied during romaine lettuce cultivation by monitoring the changes of several quality indexes at harvest and during storage. Results showed that the application of microbial inoculants during romaine lettuce cultivation contributed to the maintenance of nutritional, functional and perceived quality attributes of leaves during shelf life. The microbial inoculants prevented the development of postharvest fungal pathogen B. cinerea. Moreover, the study evidenced different modes of action of the different inoculants and, in the case of Pseudomonas syringae 260-02 application, a direct involvement of ascorbic acid-mediated antioxidant mechanisms was observed.

中文翻译：

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在栽培过程中使用微生物菌剂保持鲜切生菜的生理、营养和工艺品质

摘要 营养敏感型农业是农业食品系统中的一个新概念，它考虑实施能够保证产品的营养价值和生产的可持续性，同时减少对生态影响的技术。农业实践。这些原则也可以引入鲜切市场，目的是在保质期内保持产品质量。在这种情况下，生物基产品的使用正在迅速增加，以提高种植系统在种植和保质期内的经济和环境可持续性。这项工作的目的是评估三种不同的基于细菌的制剂（Paenibacillus pasadenensis、Bacillus amyloliquefaciens、Pseudomonas syringae) 通过监测收获和储存期间几个质量指标的变化在长叶莴苣栽培过程中应用。结果表明，在长叶莴苣栽培过程中使用微生物接种剂有助于在保质期内保持叶子的营养、功能和感知质量属性。微生物接种剂阻止了收获后真菌病原体 B. cinerea 的发展。此外，该研究证明了不同接种剂的不同作用模式，在应用丁香假单胞菌 260-02 的情况下，观察到抗坏血酸介导的抗氧化机制的直接参与。结果表明，在长叶莴苣栽培过程中使用微生物接种剂有助于在保质期内保持叶子的营养、功能和感知质量属性。微生物接种剂阻止了收获后真菌病原体 B. cinerea 的发展。此外，该研究证明了不同接种剂的不同作用模式，在应用丁香假单胞菌 260-02 的情况下，观察到抗坏血酸介导的抗氧化机制的直接参与。结果表明，在长叶莴苣栽培过程中使用微生物接种剂有助于在保质期内保持叶子的营养、功能和感知质量属性。微生物接种剂阻止了收获后真菌病原体 B. cinerea 的发展。此外，该研究证明了不同接种剂的不同作用模式，在应用丁香假单胞菌 260-02 的情况下，观察到抗坏血酸介导的抗氧化机制的直接参与。