Abstract Soybean is considered as one of the most important food for both humans and animals; unfortunately, it faces soil-borne diseases caused by bacteria or fungi, leading to severe yield losses. Hybridization has helped the soybean to obtain beneficial genes from wild species that can resist some adverse conditions. However, the influences of hybridization on the rhizosphere microbiomes of wild and cultivated soybeans have not been much elucidated until now. In this article, we investigated the influences of hybridization of wild and cultivated soybeans on the rhizosphere microbiomes using four soybean genotypes: the cultivated soybean (C), the wild soybean (W), the first hybridization generation (F1) and the second hybridization generation (F2). Results indicated that there had significant differences in both the fungal and bacterial communities in all four groups. More specifically, bacterial and fungal communities were more similar between the F2 generation and wild soybean groups. The relative abundances of Glomeromycota in wild soybean and F2 generation were significantly higher than those in C and F1 groups. Furthermore, the network analysis showed that the wild soybean had more complicated bacterial and fungal connections than other genotypes, which might help wild soybean maintain the beneficial traits of resistance to adverse conditions. Results of this study can provide a theoretical basis for recognizing the different members of the rhizosphere bacterial and fungal communities of wild and cultivated soybeans, as well as their offspring. Additionally, our findings will hopefully provide a practical guide for screening useful microbial resources from the wild soybean rhizomicrobiome.

中文翻译：

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F1和F2代杂交后野生和栽培大豆根际微生物组的组成发生了变化

摘要 大豆被认为是人类和动物最重要的食物之一；不幸的是，它面临由细菌或真菌引起的土传疾病，导致严重的产量损失。杂交帮助大豆从能够抵抗某些不利条件的野生物种中获得有益基因。然而，杂交对野生和栽培大豆根际微生物群落的影响直到现在还没有得到太多阐明。在本文中，我们使用四种大豆基因型研究了野生和栽培大豆杂交对根际微生物群落的影响：栽培大豆 (C)、野生大豆 (W)、第一代杂交 (F1) 和第二代杂交(F2)。结果表明，所有四组的真菌和细菌群落均存在显着差异。更具体地说，F2 代和野生大豆组之间的细菌和真菌群落更相似。野生大豆和F2代中Glomeromycota的相对丰度显着高于C和F1组。此外，网络分析表明，野生大豆比其他基因型具有更复杂的细菌和真菌联系，这可能有助于野生大豆保持抗逆性的有益特性。本研究结果可为识别野生和栽培大豆的根际细菌和真菌群落的不同成员及其后代提供理论基础。此外，