Abstract Third generation micro algae feedstock cultivation and fuel production have sustainable and economic benefits due to their microscopic nature. Each process in the production of micro algae biodiesel such as cultivation, harvesting and extraction can be optimized to negate the factors affecting production. Optimized micro algae biodiesel blends can be utilized to obtain ideal engine maps by calibrating engine parameters at stationary or dynamic engine tests. Numerous studies are available on micro algae based biodiesel production and its application in IC engines. Even though few consolidated articles are available on the effects of using micro algae biodiesel in IC engines, little to no article is available clubbing the production and optimization aspects of micro algae biodiesel with engine testing. To address this issue, this article intends to consolidate studies which have utilized different reactors for cultivating diverse micro algae species with multiple growth mediums. Furthermore, this combination also includes different biomass preprocessing and extraction techniques in producing micro algae biodiesel. Also, the implementation of micro algae biodiesel in IC engines and its output characteristics for different micro algae biodiesels have been highlighted and analyzed. To understand the current commercial status of micro algae biodiesel, a techno-economic analysis has been drawn. Studies revealed that closed type reactors are effective for continuous cultivation due to the constant regulation and availability of optimized growth factors. Furthermore, the techno-economic assessment revealed that the maintenance costs involved in the production should be lowered by 2–3 times the original to make micro algae biodiesel commercially available.

中文翻译：

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第三代微藻生物柴油生产及其作为未来内燃机应用生物能源的可行性的批判性审查

摘要 第三代微藻原料培养和燃料生产因其微观性质而具有可持续的经济效益。微藻生物柴油生产中的每一个过程，如培养、收获和提取，都可以优化，以消除影响生产的因素。通过在静态或动态发动机测试中校准发动机参数，可以利用优化的微藻生物柴油混合物来获得理想的发动机图。有大量关于基于微藻的生物柴油生产及其在内燃机中的应用的研究。尽管很少有关于在 IC 发动机中使用微藻生物柴油的影响的综合文章，但几乎没有文章可以将微藻生物柴油的生产和优化方面与发动机测试结合起来。为了解决这个问题，本文旨在巩固利用不同反应器培养具有多种生长介质的不同微藻物种的研究。此外，这种组合还包括生产微藻生物柴油的不同生物质预处理和提取技术。此外，还重点分析了微藻生物柴油在内燃机中的应用及其不同微藻生物柴油的输出特性。为了了解微藻生物柴油的当前商业状况，已经进行了技术经济分析。研究表明，由于优化生长因子的不断调节和可用性，封闭式反应器对于连续培养是有效的。此外，