A high-performance thin-layer chromatography method for the simultaneous quantitative determination and validation of scopoletin and gallic acid in the methanolic fraction of Jatropha glandulifera L. was developed for the first time. For achieving good separation, the mobile phase of toluene‒ethyl acetate‒glacial acetic acid (7.5:2.5:0.1, V/V) was used. Densitometric determination was carried out at 254 nm for scopoletin and gallic acid, in reflection/absorption mode; the calibration curves were linear in the range of 100‒600 ng per spot. During the analysis, the methanolic fraction of J. glandulifera L. showed the presence of scopoletin (0.08%) and gallic acid (0.04%). The proposed method is simple, precise, specific, and accurate. The obtained data can be used for the routine analysis of reported biomarkers in crude drug and extracts. The simultaneous quantification and method validation of these biomarkers have not yet been reported in J. glandulifera L. which may be utilized for the proper standardization of the plant.

首次开发了一种高效薄层色谱法，用于同时定量测定麻风树麻风树甲醇部分中的草素和没食子酸。为了获得良好的分离效果，使用了甲苯‒乙酸乙酯‒冰醋酸的流动相（7.5：2.5：0.1，V / V）。用反射/吸收模式在254 nm处对草素和没食子酸进行光密度测定。校准曲线在每个斑点100‒600 ng范围内呈线性。在分析过程中，J。glandulifera的甲醇部分L.显示存在胆碱（0.08％）和没食子酸（0.04％）。所提出的方法简单，精确，具体且准确。获得的数据可用于常规分析药物和提取物中生物标志物的常规。在J. glandulifera L.中尚未报道这些生物标记物的同时定量和方法验证，其可用于植物的适当标准化。