Glucose oxidase (GOx)-based nanotheranostic agents hold great promise in tumor starvation and its synergistic therapy. Self-assembled plasmonic gold vesicles (GVs) with unique optical properties, large hollow cavity, and strong localized surface plasmon resonance, can be used as multi-functional nanocarriers for synergistic therapy. Herein, GOx-loaded GVs (GV-GOx) were developed for light-triggered GOx release as well as enhanced catalytic activity of GOx, achieving programmable photothermal/starvation therapy. Under near-infrared laser irradiation, the GV-GOx generated strong localized hyperthermia due to plasmon coupling effect of GVs, promoting the release of encapsulated GOx and increasing its catalytic activity, resulting in enhanced tumor starvation effect. In addition, the high photothermal effect improved the cellular uptake of GV-GOx and allowed an efficient monitoring of synergistic tumor treatment via photoacoustic/photothermal duplex imaging in vivo. Impressively, the synergistic photothermal/starvation therapy demonstrated complete tumor eradication in 4T1 tumorbearing mice, verifying superior synergistic anti-tumor therapeutic effects than monotherapy with no apparent systemic side effects. Our work demonstrated the development of a light-triggered nanoplatform for cancer synergistic therapy.

基于葡萄糖氧化酶（GOx）的纳米治疗剂在肿瘤饥饿及其协同治疗中具有广阔的前景。具有独特的光学特性，大的空心腔和强烈的局部表面等离子体共振的自组装等离激元金小泡（GV）可用作多功能纳米载体进行协同治疗。本文中，开发了GOx负载GV（GV-GOx），用于光触发的GOx释放以及增强的GOx催化活性，从而实现了可编程的光热/饥饿疗法。在近红外激光照射下，GV-GOx由于GV的等离激元耦合效应而产生强烈的局部高温，从而促进了封装的GOx的释放并增加了其催化活性，从而增强了肿瘤的饥饿效果。此外，通过体内的光声/光热双工成像。令人印象深刻的是，协同光热/饥饿疗法在4T1荷瘤小鼠中显示出完全的肿瘤根除作用，证明了其比单一疗法具有更好的协同抗肿瘤治疗效果，而没有明显的系统性副作用。我们的工作证明了光触发纳米平台用于癌症协同治疗的发展。